1. Highly expressed recombinant human follicle-stimulating hormone from Chinese hamster ovary cells grown in serum-free medium and its effect on induction of folliculogenesis and ovulation 
Dong-Jae Kim, D.V.M., Seung-Hyeok Seok, Ph.D., Min-Won Baek, D.V.M., Hui-Young Lee, Ph.D., Jae-Hyeon Juhn, Ph.D., Seungwon Lee, Ph.D., Minae Yun, M.Sc., Jae-Hak Park, Ph.D. 
Fertility and Sterility 
Volume 93, Issue 8, Pages (May 2010)
DOI: /j.fertnstert
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
(A) Construction of the expression vectors (a) pBSYH-CGα-DHFR and (b) pBSYH-FSHβ-DHFR. The pLCED containing a putative internal ribosomal entry site (IRES) isolated from the encephalomyocarditis virus dihydrofolate reductase (DHFR) gene and the polyA signal of the pED vector were used for expressing rhFSH. Each synthesized gene encoding either the hCG α-subunit or the FSH β-subunit was inserted into pLCED after digestion with HindIII and XbaI, yielding pBSYH-CGα-DHFR and pBSYH-FSHβ-DHFR. (B) Matrix-associated laser desorption ionization–time of flight (MALDI-TOF) analysis results. For peptide mapping, rhFSH α-,and β-subunits were digested with trypsin for 18 hours and separated using reverse-phase high-performance liquid chromatography. The peptide map of prepared samples was analyzed using MALDI-TOF mass spectroscopy. (a) In the case of the rhFSH α-subunit, we observed fractions of most of the expected peptides (T1, T2, T4+T5, T5, and T7) and glycopeptides (N52 and N78) except for the T3 peptide, which is beyond the detection range of MALDI-TOF analysis. (b) Similarly, in the case of the rhFSH β-subunit, we observed fractions of most of the expected peptides (T4+T5, T6, T7, T8, T9, T8+T9, T9+T10, T8+T9+T10, and T11+T12) and glycopeptide (N-7) except for the N-24 glycopeptide fraction. Glycopeptides are indicated in parentheses. + = binding of other peptides.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Effect of pregnant mare serum gonadotropin (PMSG), LBFS0101, and Gonal-F on (A) ovarian weight, (B) uterine weight, and (C) number of follicles in immature hypophysectomized rats after 4 days of treatment. Results are expressed as mean ± SD. a–dThe same letter indicates a difference that was not statistically significant (P>.05). V = vehicle; P = 20 IU PMSG, L40, L20, and L5 = 40, 20, and 5 IU LBFS0101; G40, G20, and G5: 40, 20, and 5 IU Gonal-F. (D) Effect of PMSG, LBFS0101, and Gonal-F on the ovaries of immature hypophysectomized rats after 4 days of treatment. (a) vehicle; (b) 20 IU PMSG; (c–e) 40, 20, and 5 IU LBFS0101, respectively; (f–h) 40, 20, and 5 IU Gonal-F, respectively. Bar = 50 μm.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
(A) Number of oocytes collected from the oviducts after pregnant mare serum gonadotropin (PMSG)/hCG, LBFS0101/LBFS0101, Gonal-F/Gonal-F, and Follimon/Follimon treatments in androgen-sterilized mice. Results are expressed as mean ± SD. a–cThe same letter indicates a difference that was not statistically significant (P>.05). V = vehicle; PC = 5 IU PMSG/5 IU hCG; L10 and L5 = 10/10 IU and 5/5 IU LBFS010; G10 and G5 = 10/10 IU and 5/5 IU Gonal-F; F10 and F5 = 10/10 IU and 5/5 IU Follimon. (B) Ovaries after PMSG/hCG, LBFS0101/LBFS0101, and Gonal-F/Gonal-F treatment in androgen-sterilized mice. (a) vehicle; (b) 5 IU PMSG/5 IU hCG; (c) 10/10 IU LBFS010; (d) 5/5 IU LBFS010; (e) 10/10 IU Gonal-F; (f) 5/5 IU Gonal-F; (g) 10/10 IU Follimon; (h) 5/5 IU Follimon. Bar = 50 μm.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions