1. Discuss formal report assignment Get acquainted with the library
Lab 9 Goals and Objectives:
Discuss formal report assignment
Get acquainted with the library
Make new streak for isolation plate of each unknown
Finish getting Gram reaction results for each unknown
Discuss observations of the 12 possible organisms
Discuss how to rule some out
Start working on a dichotomous key of logic for solving all 12 unknowns

2. 1 2 3 4 5 6 7 8 9 10 11 12 Bacillus subtilis Enterobacter cloacae
Escherichia coli
Klebsiella pneumoniae 5 6 7 8
Proteus vulgaris
Pseudomonas aeruginosa
Salmonella typhimurium
Shigella flexnari 9 10 11 12
Staphylococcus aureus
Staphylococcus epidermidis
Enterococcus faecalis
Streptococcus pyogenes

3. The Dichotomous Key
-a visible map of logic flow
-divides a group of possible things into smaller and smaller groupings until it leads to the identification of just one
-tracing the pathway backwards shows all the characteristics that collectively are unique to only that one
-assesses only one characteristic per branch
-lines all the answers up across the bottom of the key

4. External covering: feathers External covering: hair
Example: explain with the least number of characteristics how to tell the difference between an old lady and her three pets: sort a human, a cat, a dog and a bird. Construct a Dichotomous key to ascertain the identity of each from this small group:
Animals in the house
External covering: feathers
External covering: hair
No tail
Tail
Meow
Woof
Bird
Human
Cat
Dog

5. 12 Possible Unknowns

6. 1 2 3 4 5 6 7 8 9 10 11 12 Bacillus subtilis Enterobacter cloacae
Escherichia coli
Klebsiella pneumoniae 5 6 7 8
Proteus vulgaris
Pseudomonas aeruginosa
Salmonella typhimurium
Shigella flexnari 9 10 11 12
Staphylococcus aureus
Staphylococcus epidermidis
Enterococcus faecalis
Streptococcus pyogenes

7. 12 Possible Unknowns
Gram Positive
Gram Negative

8. 12 Possible Unknowns
Gram Positive
Gram Negative

9. 12 Possible Unknowns Gram Positive Gram Negative Gelatinase +
Pseudomonas aeruginosa
Bacillus subtilis

10. 1 2 3 4 5 6 7 8 9 10 11 12 Bacillus subtilis Enterobacter cloacae
Escherichia coli
Klebsiella pneumoniae 5 6 7 8
Proteus vulgaris
Pseudomonas aeruginosa
Salmonella typhimurium
Shigella flexnari 9 10 11 12
Staphylococcus aureus
Staphylococcus epidermidis
Enterococcus faecalis
Streptococcus pyogenes