1. Volume 153, Issue 5, Pages e93-e96 (May 2018)
Severely Impaired Control of Bacterial Infections in a Patient With Cystic Fibrosis Defective in Mucosal-Associated Invariant T Cells 
Terezia Pincikova, MD, PhD, Dominic Paquin-Proulx, PhD, Markus Moll, PhD, Malin Flodström-Tullberg, PhD, Lena Hjelte, MD, PhD, Johan K. Sandberg, PhD 
CHEST 
Volume 153, Issue 5, Pages e93-e96 (May 2018)
DOI: /j.chest
Copyright © 2018 American College of Chest Physicians Terms and Conditions

2. Figure 1
Identification of a patient with CF and undetectable levels of MAIT cells in peripheral blood. PBMC samples from healthy donor (HD), the patient case (case), and matched CF patients (CF1 and CF2), were stained and analyzed for Vα7.2+CD161+ cells by flow cytometry (A). MAIT cells in PBMC from the father (F), sister (S), and the mother (M) of the patient case (B). PBMC from the various donors were pulsed with mildly fixed Escherichia coli and used to stimulate exogenously added MAIT cells from a healthy donor, and CD25 upregulation (C) and intracellular interferon-γ production (D) were measured by flow cytometry. CF = cystic fibrosis; MAIT = mucosal-associated invariant T; PBMC = peripheral blood mononuclear cells.
CHEST  , e93-e96DOI: ( /j.chest )
Copyright © 2018 American College of Chest Physicians Terms and Conditions