1. Comparative immunostaining of ATM
Comparative immunostaining of ATM. Immunoreactivity was semiquantitatively assessed with regard to staining quantity and subcellular staining intensity in 12 DPNs and 11 NMMs. Staining quantity and intensity were summarized to a single expression score (ES)...
Comparative immunostaining of ATM. Immunoreactivity was semiquantitatively assessed with regard to staining quantity and subcellular staining intensity in 12 DPNs and 11 NMMs. Staining quantity and intensity were summarized to a single expression score (ES) as recently published (7, 15, 16) and as explained in Supplementary data. Due to the strong intratumoral staining heterogeneity observed in all DPN and NMM samples, ES was always recorded for a maximum staining region (invasive tumor portion) and a minimum staining region (tumor core) per sample. To get more information about the ATM expression in relation to the complete tumor, ESmax and ESmin were proportionally summarized as EStotal. Afterward, ESmax, ESmin, and EStotal of each sample were grouped into one of five ES categories (ES 0, ES 1-50, ES , ES , and ES >150) and displayed as percentage of all samples of one entity. By this, we found a statistically significant trend to higher scores in NMMs (U test, P < 0.05).
Alexander Roesch et al. Cancer Epidemiol Biomarkers Prev 2007;16:
©2007 by American Association for Cancer Research