1. Introduction to Gel Electrophoresis

2. outline Practice gel electrophoresis
Analyze last week’s plasmid isolates
Take photograph: to be part of lab report
Interpret photograph

3. Agarose is weighed out

4. Agarose is diluted and boiled in buffer solution

5. Agarose solution is poured into gel holder

6. Agarose cools and solidifies
comb

7. Samples are added into sample wells formed by comb

8. Gel and samples are subjected to electric current

9. Samples migrate through the gel at different rates
Negative electrode
Positive electrode

10. View your DNA samples with UV light

11. Photograph the results

12. Select a micropipet from your lab station
(1000 ul= 1 ml)
Place on a tip

14. Push plunger to “first stop”
Place tip in solution
Aspirate sample by releasing plunger

15. Carefully place the tip of the micropipet just inside the well
Dispense sample by pushing to second stop
Release tip by “ejection button”

16. Practice Loading a Gel Practice with loading dye provided
Be sure an leave enough wells for your samples!
Add samples to gel
Run gel (75 v for minutes)
Photograph