1. Propionibacterium acnes catalase induces increased Th1 immune response in sarcoidosis patients 
Pariko Yorozu, Asuka Furukawa, Keisuke Uchida, Takumi Akashi, Tomoya Kakegawa, Tomohisa Ogawa, Junko Minami, Yoshimi Suzuki, Nobuyasu Awano, Haruhiko Furusawa, Yasunari Miyazaki, Naohiko Inase, Yoshinobu Eishi 
Respiratory Investigation 
Volume 53, Issue 4, Pages (July 2015)
DOI: /j.resinv
Copyright © 2015 The Japanese Respiratory Society Terms and Conditions

2. Fig. 1
Estimated accuracy of the molecular weight (MW) of the five antigenic bands detected by western blotting in a blood plasma sample obtained from a sarcoidosis patient. (A) Western blotting for P. acnes whole cell lysate (Pa) with size marker (M). The MW of each band was determined using Quantity One software. Rf, retention factor. (B) The mean (x-axis) and standard deviation (SD) (y-axis) of the estimated MW sizes for each band from three independent assays was plotted, and the curve was fitted using GraphPad Software. The minimum value of the curve was calculated to be
Respiratory Investigation  , DOI: ( /j.resinv )
Copyright © 2015 The Japanese Respiratory Society Terms and Conditions

3. Fig. 2
Detection frequency of antigenic bands detected by western blotting of blood plasma samples from sarcoidosis patients and control subjects. Each MW-sized band was detected by the secondary antibody for either human IgG (upper), IgA (middle), or IgM (lower) in 52 samples from sarcoidosis patients (black column) and 34 samples from healthy volunteers (gray column). Antigenic bands with frequencies greater than 30% in both sarcoidosis and control samples were named target bands a, b, c, d, e, f, and g.
Respiratory Investigation  , DOI: ( /j.resinv )
Copyright © 2015 The Japanese Respiratory Society Terms and Conditions

4. Fig. 3
Identification of the target antigenic bands by western blotting for each fraction separated by chromatography. Each target band, c, e, f, or g, was identified by western blotting for P. acnes whole cell lysate (left lane) and for a fractionated sample containing the antigen (middle lane) and by SDS-PAGE stained for proteins (right lane). Each target antigenic protein band is indicated by an arrowhead.
Respiratory Investigation  , DOI: ( /j.resinv )
Copyright © 2015 The Japanese Respiratory Society Terms and Conditions

5. Fig. 4
Immune responses to the three recombinant proteins of mice immunized with P. acnes whole cell lysate. (A) Antibody titers were measured by ELISA with serum samples from P. acnes-immunized mice (n=41) and control mice (n=36). (B) Th1 immune responses were measured using an ELISPOT IFN-γ assay with lymph node cells from P. acnes-immunized mice (n=10) and control mice (n=10). *Mann–Whitney U test. OD490, optical density at 490 nm; PA, P. acnes-immunized mice; CNT, control mice; ADI, arginine deiminase; KAT, catalase; UAP, UDP-N-acetylglucosamine pyrophosphorylase.
Respiratory Investigation  , DOI: ( /j.resinv )
Copyright © 2015 The Japanese Respiratory Society Terms and Conditions

6. Fig. 5
IgG, IgA, or IgM titers to the three recombinant proteins of sarcoidosis patients and control subjects. Antibody titers were measured by ELISA with blood plasma samples from sarcoidosis patients (n=52) and healthy volunteers (n=34). ⁎Mann–Whitney U test. OD490, optical density at 490 nm; SA, sarcoidosis patients; HV, healthy volunteers; ADI, arginine deiminase; KAT, catalase; UAP, UDP-N-acetylglucosamine pyrophosphorylase; N.S., not significant.
Respiratory Investigation  , DOI: ( /j.resinv )
Copyright © 2015 The Japanese Respiratory Society Terms and Conditions

7. Fig. 6
Th1 immune responses to the three recombinant proteins of sarcoidosis patients and control subjects. Responses were measured using an ELISPOT IFN-γ assay with peripheral blood mononuclear cells from sarcoidosis patients (n=12), other pneumonitis patients (n=13), and healthy volunteers (n=11). *Kruskal–Wallis test, **Dunn’s multiple test. SA, sarcoidosis patients; OP, other pneumonitis patients; HV, healthy volunteers; ADI, arginine deiminase; KAT, catalase; UAP, UDP-N-acetylglucosamine pyrophosphorylase; N.S., not significant.
Respiratory Investigation  , DOI: ( /j.resinv )
Copyright © 2015 The Japanese Respiratory Society Terms and Conditions