1. Differential expression of vascular endothelial growth factor-A isoforms in the mouse uterus during early pregnancy 
Lisa M. Walter, Peter A.W. Rogers, Jane E. Girling 
Reproductive BioMedicine Online 
Volume 21, Issue 6, Pages (December 2010)
DOI: /j.rbmo
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2. Figure 1
Uterine vascular endothelial growth factor (VEGF)-A isoform mRNA expression in CBA/C57 mice from day 1 to 5 of pregnancy. The mRNA expression of (A) total Vegf-A, (B) Vegf120, (C) Vegf164 and (D) Vegf188 in pregnant mice was normalized against 18S rRNA. Data is expressed as the fold change compared with the median value on day 1 of pregnancy. The lines in the boxes represent the medians and the boxes extend from the 25th to the 75th centiles. The error bars indicate the minimum and maximum values (n=6). Groups that do not share a letter in common are significantly different (P<0.05).
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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3. Figure 2
Uterine vascular endothelial growth factor (VEGF)-A receptor mRNA expression in CBA/C57 mice from day 1 to 5 of pregnancy. The mRNA expression of (A) Vegfr-2, (B) Nrp-1 and (C) Nrp-2 in pregnant mice were normalized against 18S rRNA. Data is expressed as the fold change compared with the median value on day 1 of pregnancy. The lines in the boxes represent the medians and the boxes extend from the 25th to the 75th centiles. The error bars indicate the minimum and maximum values (n=6). Groups that do not share a letter in common are significantly different (P<0.05).
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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4. Figure 3
Uterine vascular endothelial growth factor (VEGF)-A protein expression in CBA/C57 mice from day 1 (D1) to 5 (D5) of pregnancy. Representative Western blot analysis of VEGF-A isoforms under reducing conditions. Bands at 20–23kDa and 27kDa represent VEGF164 and VEGF188, respectively. A very pale band was observed at ∼18kDa in some samples (thought to be VEGF120, white arrows). Molecular weight of bands determined by comparison with pre-stained, broad range standards (left-hand side). BC=Breast cancer sample used as positive control.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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5. Figure 4
Vascular endothelial growth factor receptor (VEGFR)-2 immunostaining in CBA/C57 mouse uterus during early pregnancy. Representative photomicrograph of endometrium from pregnant CBA/C57 mice immunostained with VEGFR-2 antibody. Inset; negative control (rabbit IgG). Black arrows indicate blood vessels; e=luminal epithelium; g=gland; l=uterine lumen; s=stroma. Bar=100 μm.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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