1. RNA Synthesis
Based on LNC Ch 26
Title slide

2. The overall scheme of transcription.

3. The winding & unwinding of DNA.

4. Relationship between DNA strands and product RNA.

5. Which strand - both, it just depends.

6. The structure of E. coli RNA polymerase.

7. Consensus signal sequences recognized by RNA polymerase.

8. The steps in initiating RNA synthesis.

9. Protein required for eukaryotic RNA polymerase II.

10. After synthesis the mRNA is spliced, capped and tailed.

11. The detailed reactions.

12. Caps and modifications.

13. Capping.

14. Tailing.