1. DO NOW QUESTION
Do you think it is ok for humans to modify genes in living organisms? Why or Why not?
Campbell 8thed Chapter 20/ Lemur Chapter 12

2. SWBAT describe technological advances using DNA and the genes.
Objective Date:
SWBAT describe technological advances using DNA and the genes.

3. In CSI: Waukegan, we discussed how biological fluids can be used to find DNA of a suspect.
DNA fingerprinting allows us to confirm identifies based on an individual's unique sequence.
Defendant’s
blood
Blood from
defendant’s clothes
Victim’s

4. BACTERIAL PLASMIDS AND GENE CLONING
Plasmids are used to customize bacteria.
We can move the plasmid into other cells.

5. BACTERIAL PLASMIDS AND GENE CLONING
Uses:
Pest Resistant Plants
Cleaning up toxic waste
Dissolving blood clots
Human Growth Hormone & Insulin

6. CONNECTION

7. In 1982, humulin, human insulin was produced by bacteria
Therapeutic hormones
In 1982, humulin, human insulin was produced by bacteria
Became the first recombinant drug approved by the Food and Drug Administration (FDA)
Figure 12.7A

8. Diagnosis and Treatment of Disease1 2 3 4 5
Restriction fragment
preparation
Gel electrophoresis
Blotting
Radioactive probe
Detection of radioactivity
(autoradiography) I II
III
Restriction
fragments
Filter paper
Probe
Radioactive, single-
stranded DNA (probe)
Film
Diagnosis and Treatment of Disease
Recognize dangerous inherited diseases

9. Gene therapy may someday help treat a variety of diseases
CONNECTION
Gene therapy may someday help treat a variety of diseases
Cloned gene
(normal allele) 1
Insert normal gene
into virus
Viral nucleic
acid
Retrovirus 2
Infect bone marrow
cell with virus 3
Viral DNA inserts
into chromosome
Bone marrow
cell from patient
Bone
marrow
Figure 12.13 4
Inject cells
into patient

10. Pharm Animals
Genes from humans are moved into another species creating a transgenic animal.
Goats have been used to create the human blood protein (antithrombin) for people who cannot create blood clots.

11. Bioengineering & Farm Animals

12. The Human Genome Project, begun in 1990 and completed in 2003.
GENOMICS CONNECTION
The Human Genome Project, begun in 1990 and completed in 2003.
Genetic and physical mapping of chromosomes, followed by DNA sequencing
Figure 12.15

13. Besides being interesting themselves
Table 12.17
Besides being interesting themselves
Nonhuman genomes can be compared with the human genome

14. GENETICALLY MODIFIED ORGANISMS and FOOD
Genetically modified organisms (GMOs) are transforming agriculture

15. Super Salmon ABC News

16. How do you know what you are eating?

17. Genetic engineering involves risks
Genetic engineering involves risks
Such as ecological damage from GM crops
Figure 12.19B

18. PROS CONS People have always selectively breed. Strictly Monitored
More diversity
Crops can grow in scarce land and without use of pesticides.
Cheaper food & medicine
Unnatural
May not know the issues for a long time
Uses bacteria and viruses to transfer genes
Pesticides used will kill all non-GMOs.
Will not solve world hunger