1. DNA and Chromosome Replication
Lecture BSCI 420/421/620 Sept.23, 2002
“Only after the last tree has been cut down. Only after the
last river has been poisoned. Only after the last fish has
been caught. Only then will you find that money cannot
be eaten.” - Cree Indian Prophecy
A. Prokaryotic chromosome replication
B. DNA synthesis
C. Initiation of DNA replication

2. A. Prokaryotic and organellar chromosome replication
1. These are circular molecules w few proteins
2. 2 specialized regions: a. One origin of replication
b. Membrane attachment point. Same? Yes

3. B. DNA Replication

6. DNA primase

7. Lagging strand synthesis

8. DNA helicase

9. Single-strand binding proteins

10. Replication fork in eucaryotes

11. Moving replication fork

12. DNA topoisomerases prevent DNA tangling during replication or
translation

13. Topoisomerase 1

14. Topoisomerase II

15. C. Initiation of DNA Replication
Begins at Origins of Replication

18. Eukaryotic (yeast) ORI genes

19. New nucleosome
assembly