1. Warm Up Which of the following is a nucleotide found in DNA?
a. ribose + phosphate group + thymine
b. ribose + phosphate group + uracil
c. deoxyribose + phosphate group + uracil
d. deoxyribose + phosphate group + cytosine

2. Warm Up Which of the following is a nucleotide found in DNA?
a. ribose + phosphate group + thymine
b. ribose + phosphate group + uracil
c. deoxyribose + phosphate group + uracil
d. deoxyribose + phosphate group + cytosine

3. DNA Structure & Replication

4. Figure 12–1 shows the structure of a(an)
a.DNA molecule.
b.RNA molecule.
c.amino acid.
d.protein.

5. Figure 12–1 shows the structure of a(an)
a.DNA molecule.
b.RNA molecule.
c.amino acid.
d.protein.

6. Let’s Review

7. What is DNA? Deoxyribonucleic Acid
DNA is the molecule involved in inheritance.
All the genetic information (instructions) that are used to build your body.

8. nucleotides Nucleotides are the monomers of nucleic acids.
Nucleotides are made of: 5 carbon sugar, phosphate group, and a nitrogenous base

9. Nitrogenous Bases 4 Nitrogenous Bases: Adenine (A) Thymine (T)
Guanine (G)
Cytosine (C)
Pyrimidines- single rings
Purines- double rings

10. Base pairing Adenine pairs with Thymine (A-T)
Cytosine pairs with Guanine (C-G)

11. DNA Structure Shaped like a double helix
DNA is compared to a twisted ladder!
The steps of the ladder are the paired bases.
The rails are sugars and phosphates that alternate.

12. What does DNA look like in living cells?
Let’s extract DNA from some common fruits that we eat so we can see.

13. You will be given an “template Stand, create the complementary strand .
Opening

14. DNA REPLICATION

15. Work Session
DNA Coloring:
Handout
DNA History Webquest

16. Semiconservative replication
Parent strands of DNA separate and serve as the template for the new DNA.
Each DNA molecule has a parent strand and a new one!
Occurs during interphase of mitosis and meiosis.

17. Antiparallel DNA strands run “antiparallel” to one another.
The 5’ (five prime) end has a phosphate group.
The 3’ (three prime) end has a sugar.

18. Step 1:
HELICASE unwinds a segment of DNA
It “unzips” the double helix

19. Step 2:
RNA Primase attaches to the DNA and synthesizes many short RNA primers

20. Step 3:
DNA Polymerase III adds new nucleotides to the 3’ end of the RNA primer

21. Leading vs. lagging strands
Leading Strand
Synthesized continuously from 5’ to 3’
Lagging Strand (Okazaki fragments)
Since DNA Polymerase III can only add new nucleotides to the 3’ end of the primer, this strand is synthesized DIScontinuously

22. Step 4:
DNA Polymerase I will then remove the RNA primers and replace them with DNA nucleotides

23. Step 5:
DNA Ligase is a “linking” enzyme that seals the Okazaki fragments together

24. DNA Replication
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