1. Agilent 1100 Series System Operating principles
Pumping principle
Sampler principles
TCC peltier principle
Detector principle
Introduction
11/13/2018
ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

2. Agilent 1100 Series Pump Principles
Explain how the pump is designed and where the different components are situated.
11/13/2018
ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

3. Agilent 1100 Series Pump Principle
Explain the differences between the G1311A and G1312A and the difference in delay volume and mixing performance. Stress where the first point of mixing are in the two pumps.
11/13/2018
ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

4. Agilent 1100 Series Pump Principle
LPM Board
Common to Iso and Quat Pumps ("Type"? )
Metering Drive
Power Supply
1100 Common Part
Fan
Flow Pulse Damper
Common to 1100, HP1050
and HP1090 Pumps
Point to the different components of the pump.
Pump Head
Leak Sensor
1100 Common Part
Leak Tray
11/13/2018
ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

5. Agilent 1100 Series Autosampler Principle
Describe the injection cycle. BEWARE the slide is wrong. The line from the valve to the metering should go into the metering head instead.
11/13/2018
ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

6. Agilent 1100 Series Autosampler Principle
Critical Connections, snap down correctly!
ASM Board
Transport Unit
Power Supply
Common 1100 Part
Sampling Unit
Point to the different parts of the sampler.
Vial Trays: 100-2ml
40-2ml
15-ml
11/13/2018
ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

7. Agilent 1100 Series Well Plate Sampler principle
Explain the difference of the cycle compared to the Standard sampler.
11/13/2018
ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

8. Agilent 1100 Series Thermostatted Column Compartment principle
Explain the Peltier principle and the use of the two different sides.
11/13/2018
ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

9. Agilent 1100 Series Variable Wavelength Detector Principle
Lamp
Optical mirrors
Grating
Detector cell
Slit
Beam splitter
Explain how the G1314A measures a peak and how the referencing is done.
11/13/2018
ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

10. Agilent 1100 Series Variable Wavelength Detector Principle
VWD Board
Lamp housing
Fan
Power Supply
1100 Common Part
Point to the different parts of the VWD.
Optical unit
Flow cell
Leak Sensor
1100 Common Part
11/13/2018
ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

11. Recommended Flow Cells
For Pressure
Above 100 bar
Explain how to read this slide and why the column ID and flow is interconnected.
11/13/2018
ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

12. Agilent 1100 Series Diode Array Detector Principle
Tungsten lamp
Lens
Deuterium lamp
Lens system
Holmium filter
Detector cell
Optical slit
Grating
Diode Array
Explain how the G1315B measures a peak and how the referencing is done. Explain the difference between the A and the B.
11/13/2018
ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

13. Agilent 1100 Series Diode Array Detector Principle
DAD Board
Fan
Lamp housing
Power Supply
1100 Common Part
Point to the different parts of the DAD.
Optical unit
Flow cell
Leak Sensor
1100 Common Part
11/13/2018
ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

14. Recommended Flow cells
See previous slide.
11/13/2018
ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction