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Evaluation of the Epstein-Barr Virus R-Gene Quantification Kit in Whole Blood with Different Extraction Methods and PCR Platforms  Samira Fafi-Kremer,

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Presentation on theme: "Evaluation of the Epstein-Barr Virus R-Gene Quantification Kit in Whole Blood with Different Extraction Methods and PCR Platforms  Samira Fafi-Kremer,"— Presentation transcript:

1 Evaluation of the Epstein-Barr Virus R-Gene Quantification Kit in Whole Blood with Different Extraction Methods and PCR Platforms  Samira Fafi-Kremer, Patrice Morand, Come Barranger, Gérard Barguès, Stéphane Magro, Jérôme Bés, Philippe Bourgeois, Martine Joannes, Jean-Marie Seigneurin  The Journal of Molecular Diagnostics  Volume 10, Issue 1, Pages (January 2008) DOI: /jmoldx Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Analytic sensitivity of the EBV R-gene assay with two different PCR platforms. The EBV R-gene assay used with both PCR platforms, after manual extraction, detected all six replicates of specimen containing 10 copies of EBV DNA per reaction. The Journal of Molecular Diagnostics  , 78-84DOI: ( /jmoldx ) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 Correlation of EBV DNA load quantified in EBV-positive whole-blood samples (n = 126) by the EBV R-gene quantification kit in the SmartCycler 2.0 and in-house PCR in the LightCycler 1.0, after automated extraction by the MagNAPure LC (r = 0.92; P < ). The Journal of Molecular Diagnostics  , 78-84DOI: ( /jmoldx ) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

4 Figure 3 Correlation of longitudinal EBV DNA load dynamics measured by the EBV R-gene quantification kit and in-house PCR in two patients at risk for EBV-associated diseases (patients A and B). GVHD, graft-versus-host reaction disease; ATG, antithymocyte globulin. The Journal of Molecular Diagnostics  , 78-84DOI: ( /jmoldx ) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

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