1. DNA Purification and Analysis

7. The Southern Blot Technique: -technique for DNA analysis developed in the 1970s by E. M. Southern -Southern Blot allows detection and determination of the number of copies of particular sequences in complex DNA mixtures

8. The detection of DNAs containing specific base sequences by Southern Blotting

9. -Restriction endonucleases are capable of selectively dissecting DNA molecules into smaller fragments -Restriction endonucleases permit construction of a new type of genetic map, a restriction map, in which the site of enzyme cleavage within the DNA is identified -A comparison of restriction nucleases sites in mitochondrial DNA (mammalian mitochondrial DNA is a circular DNA molecule of 16,569 bp) allows for the calculation of the degree of divergence in nucleotide sequence between species

17. The Sanger Procedure of DNA Sequencing -is based on the random termination of a DNA chain during enzymatic DNA synthesis -the technique is possible because the dideoxynucleotide analog of each of the four normal nucleotides can be incorporated into a growing DNA chain by DNA polymerase