2. Science Learning Center Chemistry Pre-laboratory Tutorials
Written By Patrick Herklotz

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4. Calibrating and Operating the Vernier pH Sensor
Tutorial 4
Calibrating and Operating the Vernier pH Sensor

5. Calibrating and Operating the Vernier pH Sensor
Tutorial 4
Calibrating and Operating the Vernier pH Sensor
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6. Step 1: Assemble the LabPro Calculator as explained in Tutorial 1
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Step 1: Assemble the LabPro Calculator as explained in Tutorial 1
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Top View
Power Supply
LabPro
VERNIER
Quick Setup
Start/Stop
Transfer
Vernier
STAT PLOT F1
TBLSET F2
FORNAT F3
CALC F4
TABLE F5
Alpha
2ND
Mode
DEL
STAT
PRGM
COS (
X,T,O,N
APPS
MATH 7 4 1 8 5 2 . 9 6 3
(-) x + - ÷
Enter
X -1 X2
LOG LN
STO> ON
SIN ,
VARS
CLEAR
TAN
> )

7. Step 1: Assemble the LabPro Calculator as explained in Tutorial 1.
Previous Slide
Step 1: Assemble the LabPro Calculator as explained in Tutorial 1.
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Top View
Power Supply
LabPro
VERNIER
Quick Setup
Start/Stop
Transfer
Vernier
STAT PLOT F1
TBLSET F2
FORNAT F3
CALC F4
TABLE F5
Alpha
2ND
Mode
DEL
STAT
PRGM
COS (
X,T,O,N
APPS
MATH 7 4 1 8 5 2 . 9 6 3
(-) x + - ÷
Enter
X -1 X2
LOG LN
STO> ON
SIN ,
VARS
CLEAR
TAN
> )

8. Previous Slide
Step 2: Obtain the pH sensor and plug it into channel 1. Run the DataMate program by selecting it from the APPS menu in order to allow the calculator to detect the pH sensor.
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Top View
Power Supply
LabPro
VERNIER
Quick Setup
Start/Stop
Transfer
Vernier
STAT PLOT F1
TBLSET F2
FORNAT F3
CALC F4
TABLE F5
Alpha
2ND
Mode
DEL
STAT
PRGM
COS (
X,T,O,N
APPS
MATH 7 4 1 8 5 2 . 9 6 3
(-) x + - ÷
Enter
X -1 X2
LOG LN
STO> ON
SIN ,
VARS
CLEAR
TAN
> )
Channel 1
Channel 2
Channel 3
Channel 4
Side View

9. Previous Slide
Step 2: Obtain the pH sensor and plug it into channel 1. Run the DataMate program by selecting it from the APPS menu in order to allow the calculator to detect the pH sensor.
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Top View
Power Supply
pH SENSOR
LabPro
VERNIER
Quick Setup
Start/Stop
Transfer
Vernier
STAT PLOT F1
TBLSET F2
FORNAT F3
CALC F4
TABLE F5
Alpha
2ND
Mode
DEL
STAT
PRGM
COS (
X,T,O,N
APPS
MATH 7 4 1 8 5 2 . 9 6 3
(-) x + - ÷
Enter
X -1 X2
LOG LN
STO> ON
SIN ,
VARS
CLEAR
TAN
> )
VERNIER SOFTWARE
DATAMATE
(VER 1.15)
ROM (C)2003
Checking Sensors
Mode: TIME GRAPH-180
1: SETUP : ANALYZE
2: START : TOOLS
3: GRAPH : QUIT
CH 1: Absorbance
Mode: TIME GRAPH-180
1: SETUP : ANALYZE
2: START : TOOLS
3: GRAPH : QUIT
click
Channel 1
Channel 2
Channel 3
Channel 4
Channel 1 Port
Side View

10. LabPro Calculator Screen
Previous Slide
Step 3: In the default DataMate program screen, press the 1 key (SETUP). In the next menu, press 2 (CALIBRATE) once the cursor triangle is to the left of CH 1. In the third menu, press the 2 key (CALIBRATE NOW).
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Power Supply
pH SENSOR
LabPro
VERNIER
Quick Setup
Start/Stop
Transfer
Vernier
STAT PLOT F1
TBLSET F2
FORNAT F3
CALC F4
TABLE F5
Alpha
2ND
Mode
DEL
STAT
PRGM
COS (
X,T,O,N
APPS
MATH 7 4 1 8 5 2 . 9 6 3
(-) x + - ÷
Enter
X -1 X2
LOG LN
STO> ON
SIN ,
VARS
CLEAR
TAN
> )
Texas Instruments
TI-84 Silver Edition
CH 1: PH
Mode: TIME GRAPH-180
1: SETUP 4: ANALYZE
2: START 5: TOOLS
3: GRAPH 6: QUIT
LabPro Calculator Screen

11. LabPro Calculator Screen
Previous Slide
Step 3: In the default DataMate program screen, press the 1 key (SETUP). In the next menu, press 2 (CALIBRATE) once the cursor triangle is to the left of CH 1. In the third menu, press the 2 key (CALIBRATE NOW).
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Continue
Power Supply
pH SENSOR
LabPro
VERNIER
Quick Setup
Start/Stop
Transfer
Vernier
STAT PLOT F1
TBLSET F2
FORNAT F3
CALC F4
TABLE F5
Alpha
2ND
Mode
DEL
STAT
PRGM
COS (
X,T,O,N
APPS
MATH 7 4 1 8 5 2 . 9 6 3
(-) x + - ÷
Enter
X -1 X2
LOG LN
STO> ON
SIN ,
VARS
CLEAR
TAN
> )
Texas Instruments
TI-84 Silver Edition
CH 1: PH
Mode: TIME GRAPH-180
1: SETUP 4: ANALYZE
2: START 5: TOOLS
3: GRAPH 6: QUIT
CALIBRATION SENSOR
MONITOR VOLTAGE, WHEN
STABLE,PRESS [ENTER].
VALUE VOLTAGE
POINT 1:
POINT 2:
CALIBRATION PH
CALIBRATION: LINEAR
SLOPE INT
1:OK
2:CALIBRATE NOW
3:MANUAL ENTRY
CH 1: PH
CH 2:
CH 3:
CH 4:
DIG1:
DIG2:
MODE: TIME GRAPH-900
1:OK :ZERO
2:CALIBRATE :SAVE/LOAD
LabPro Calculator Screen

12. LabPro Calculator Screen
Step 4: Remove the pH sensor from the solution bottle that keeps it moist. Thoroughly rinse the pH electrode with DI water over a beaker. Always perform this step before measuring the pH of a new solution. Use a Chemwipe to gently rub off strong bases from the sensor’s electrode after rinsing it with DI water.
Previous Slide
Replay
Continue -
Power Supply
LabPro
VERNIER
Quick Setup
Start/Stop
Transfer
Vernier
STAT PLOT F1
TBLSET F2
FORNAT F3
CALC F4
TABLE F5
Alpha
2ND
Mode
DEL
STAT
PRGM
COS (
X,T,O,N
APPS
MATH 7 4 1 8 5 2 . 9 6 3
(-) x + - ÷
Enter
X -1 X2
LOG LN
STO> ON
SIN ,
VARS
CLEAR
TAN
> )
pH SENSOR 50
100
150
250
300
0 ml
Texas Instruments
TI-84 Silver Edition
CALIBRATION SENSOR
MONITOR VOLTAGE, WHEN
STABLE,PRESS [ENTER].
VALUE VOLTAGE
POINT 1:
POINT 2:
LabPro Calculator Screen

13. LabPro Calculator Screen
Step 4: Remove the pH sensor from the solution bottle that keeps it moist. Thoroughly rinse the pH electrode with DI water over a beaker. Always perform this step before measuring the pH of a new solution. Use a Chemwipe to gently rub off strong bases from the sensor’s electrode after rinsing it with DI water.
Previous Slide
Replay
Continue -
Power Supply
LabPro
VERNIER
Quick Setup
Start/Stop
Transfer
Vernier
STAT PLOT F1
TBLSET F2
FORNAT F3
CALC F4
TABLE F5
Alpha
2ND
Mode
DEL
STAT
PRGM
COS (
X,T,O,N
APPS
MATH 7 4 1 8 5 2 . 9 6 3
(-) x + - ÷
Enter
X -1 X2
LOG LN
STO> ON
SIN ,
VARS
CLEAR
TAN
> )
pH SENSOR
Rinse pH electrode with DI water from lab squeeze bottles. 50
100
150
250
300
0 ml
Texas Instruments
TI-84 Silver Edition
CALIBRATION SENSOR
MONITOR VOLTAGE, WHEN
STABLE,PRESS [ENTER].
VALUE VOLTAGE
POINT 1:
POINT 2:
LabPro Calculator Screen

14. LabPro Calculator Screen
Step 5: Place the sensor electrode into a buffer that has a pH of 4. Allow the voltage to stabilize to a reasonably constant value out to the hundredth place past the decimal. This may take seconds. Once this occurs, press the ENTER key. On the next screen, press the 4 key to input the pH of this buffer into the calculators memory. Press the ENTER Key again.
Previous Slide
Replay
Continue -
Power Supply
LabPro
VERNIER
Quick Setup
Start/Stop
Transfer
Vernier
STAT PLOT F1
TBLSET F2
FORNAT F3
CALC F4
TABLE F5
Alpha
2ND
Mode
DEL
STAT
PRGM
COS (
X,T,O,N
APPS
MATH 7 4 1 8 5 2 . 9 6 3
(-) x + - ÷
Enter
X -1 X2
LOG LN
STO> ON
SIN ,
VARS
CLEAR
TAN
> )
pH SENSOR 50
100
150
250
300
0 ml
pH 4 Buffer
Texas Instruments
TI-84 Silver Edition
CALIBRATION SENSOR
MONITOR VOLTAGE, WHEN
STABLE,PRESS [ENTER].
VALUE VOLTAGE
POINT 1:
POINT 2:
LabPro Calculator Screen

15. LabPro Calculator Screen
Step 5: Place the sensor electrode into a buffer that has a pH of 4. Allow the voltage to stabilize to a reasonably constant value out to the hundredth place past the decimal. This may take seconds. Once this occurs, press the ENTER key. On the next screen, press the 4 key to input the pH of this buffer into the calculators memory. Press the ENTER Key again.
Previous Slide
Replay
Continue -
Power Supply
LabPro
VERNIER
Quick Setup
Start/Stop
Transfer
Vernier
STAT PLOT F1
TBLSET F2
FORNAT F3
CALC F4
TABLE F5
Alpha
2ND
Mode
DEL
STAT
PRGM
COS (
X,T,O,N
APPS
MATH 7 4 1 8 5 2 . 9 6 3
(-) x + - ÷
Enter
X -1 X2
LOG LN
STO> ON
SIN ,
VARS
CLEAR
TAN
> )
pH SENSOR 50
100
150
250
300
0 ml
pH 4 Buffer
Texas Instruments
TI-84 Silver Edition
CALIBRATION SENSOR
MONITOR VOLTAGE, WHEN
STABLE,PRESS [ENTER].
VALUE VOLTAGE
POINT 1:
POINT 2: 1
ENTER VALUE ?
CALIBRATION SENSOR
MONITOR VOLTAGE, WHEN
STABLE,PRESS [ENTER].
VALUE VOLTAGE
POINT 1:
POINT 2: 4
LabPro Calculator Screen
1.635
1.729
1.631

16. LabPro Calculator Screen
Step 6: Rinse the electrode with DI water. Next, place the pH electrode into a buffer that has a pH of 7. Allow the voltage to stabilize to a reasonably constant value out to the hundredths place past the decimal. Once this occurs, press the ENTER key. On the next screen, press the 7 key to input the pH of this buffer into the calculators memory. Press ENTER to complete the calibrating process.
Previous Slide
Replay
Continue -
Power Supply
LabPro
VERNIER
Quick Setup
Start/Stop
Transfer
Vernier
STAT PLOT F1
TBLSET F2
FORNAT F3
CALC F4
TABLE F5
Alpha
2ND
Mode
DEL
STAT
PRGM
COS (
X,T,O,N
APPS
MATH 7 4 1 8 5 2 . 9 6 3
(-) x + - ÷
Enter
X -1 X2
LOG LN
STO> ON
SIN ,
VARS
CLEAR
TAN
> )
pH SENSOR 50
100
150
250
300
0 ml
pH 7 Buffer
Texas Instruments
TI-84 Silver Edition
CALIBRATION SENSOR
MONITOR VOLTAGE, WHEN
STABLE,PRESS [ENTER].
VALUE VOLTAGE
POINT 1:
POINT 2:
LabPro Calculator Screen

17. The pH sensor is now calibrated! LabPro Calculator Screen
Step 6: Rinse the electrode with DI water. Next, place the pH electrode into a buffer that has a pH of 7. Allow the voltage to stabilize to a reasonably constant value out to the hundredths place past the decimal. Once this occurs, press the ENTER key. On the next screen, press the 7 key to input the pH of this buffer into the calculators memory. Press ENTER to complete the calibrating process.
Previous Slide
Replay
Continue -
Power Supply
LabPro
VERNIER
Quick Setup
Start/Stop
Transfer
Vernier
STAT PLOT F1
TBLSET F2
FORNAT F3
CALC F4
TABLE F5
Alpha
2ND
Mode
DEL
STAT
PRGM
COS (
X,T,O,N
APPS
MATH 7 4 1 8 5 2 . 9 6 3
(-) x + - ÷
Enter
X -1 X2
LOG LN
STO> ON
SIN ,
VARS
CLEAR
TAN
> )
pH SENSOR 50
100
150
250
300
0 ml
The pH sensor is now calibrated!
pH 7 Buffer
Texas Instruments
TI-84 Silver Edition
CALIBRATION PH
CALIBRATION: LINEAR
SLOPE INT
1:OK
2:CALIBRATE NOW
3:MANUAL ENTRY 1
ENTER VALUE ?
CALIBRATION SENSOR
MONITOR VOLTAGE, WHEN
STABLE,PRESS [ENTER].
VALUE VOLTAGE
POINT 1:
POINT 2:
CALIBRATION SENSOR
MONITOR VOLTAGE, WHEN
STABLE,PRESS [ENTER].
VALUE VOLTAGE
POINT 1:
POINT 2: 7
LabPro Calculator Screen
3.971
4.452
3.976

18. Step 7: Press the 1 key [OK] to return to the Settings menu
Step 7: Press the 1 key [OK] to return to the Settings menu. Press the 1 key [OK] again to return to the main menu.
Previous Slide
Replay
Continue -
Power Supply
LabPro
VERNIER
Quick Setup
Start/Stop
Transfer
Vernier
STAT PLOT F1
TBLSET F2
FORNAT F3
CALC F4
TABLE F5
Alpha
2ND
Mode
DEL
STAT
PRGM
COS (
X,T,O,N
APPS
MATH 7 4 1 8 5 2 . 9 6 3
(-) x + - ÷
Enter
X -1 X2
LOG LN
STO> ON
SIN ,
VARS
CLEAR
TAN
> )
pH SENSOR 50
100
150
250
300
0 ml
Texas Instruments
TI-84 Silver Edition
CALIBRATION PH
CALIBRATION: LINEAR
SLOPE INT
1:OK
2:CALIBRATE NOW
3:MANUAL ENTRY

19. pH reading for a solution
Step 7: Press the 1 key [OK] to return to the Settings menu. Press the 1 key [OK] again to return to the main menu.
Previous Slide
Replay
Continue -
Power Supply
LabPro
VERNIER
Quick Setup
Start/Stop
Transfer
Vernier
STAT PLOT F1
TBLSET F2
FORNAT F3
CALC F4
TABLE F5
Alpha
2ND
Mode
DEL
STAT
PRGM
COS (
X,T,O,N
APPS
MATH 7 4 1 8 5 2 . 9 6 3
(-) x + - ÷
Enter
X -1 X2
LOG LN
STO> ON
SIN ,
VARS
CLEAR
TAN
> )
pH SENSOR 50
100
150
250
300
0 ml
Texas Instruments
TI-84 Silver Edition
CALIBRATION PH
CALIBRATION: LINEAR
SLOPE INT
1:OK
2:CALIBRATE NOW
3:MANUAL ENTRY
CH 1: PH
CH 2:
CH 3:
CH 4:
DIG1:
DIG2:
MODE: TIME GRAPH-900
1:OK :ZERO
2:CALIBRATE :SAVE/LOAD
CH 1: PH
Mode: TIME GRAPH-180
1: SETUP 4: ANALYZE
2: START 5: TOOLS
3: GRAPH 6: QUIT
pH reading for a solution

20. Calibrating The pH Sensor: Review of Steps 5-7
Previous Slide
Calibrating The pH Sensor: Review of Steps 5-7
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Texas Instruments
Texas Instruments
Texas Instruments
TI-84 Silver Edition
TI-84 Silver Edition
TI-84 Silver Edition
CALIBRATION PH
CALIBRATION: LINEAR
SLOPE INT
1:OK
2:CALIBRATE NOW
3:MANUAL ENTRY
CALIBRATION SENSOR
MONITOR VOLTAGE, WHEN
STABLE,PRESS [ENTER].
VALUE VOLTAGE
POINT 1:
POINT 2: 1
ENTER VALUE
? 4
(1) Press 2: CALIBRATE NOW
(3) Press 4 Key then the ENTER Key
(2) Press ENTER key
Texas Instruments
Texas Instruments
Texas Instruments
TI-84 Silver Edition
TI-84 Silver Edition
TI-84 Silver Edition
CALIBRATION SENSOR
MONITOR VOLTAGE, WHEN
STABLE,PRESS [ENTER].
VALUE VOLTAGE
POINT 1:
POINT 2: 2
ENTER VALUE
? 7
CALIBRATION PH
CALIBRATION: LINEAR
SLOPE INT
1:OK
2:CALIBRATE NOW
3:MANUAL ENTRY
(5) Press 7 key then the ENTER key
(6) Press 1 twice to return to default menu screen
(4) Press ENTER key

21. Calibrating The pH Sensor: Review of Steps 5-7
Previous Slide
Calibrating The pH Sensor: Review of Steps 5-7
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Continue
Texas Instruments
Texas Instruments
Texas Instruments
TI-84 Silver Edition
TI-84 Silver Edition
TI-84 Silver Edition
CALIBRATION PH
CALIBRATION: LINEAR
SLOPE INT
1:OK
2:CALIBRATE NOW
3:MANUAL ENTRY
CALIBRATION SENSOR
MONITOR VOLTAGE, WHEN
STABLE,PRESS [ENTER].
VALUE VOLTAGE
POINT 1:
POINT 2: 1
ENTER VALUE
? 4
(1) Press 2: CALIBRATE NOW
(3) Press 4 Key then the ENTER Key
(2) Press ENTER key
Texas Instruments
Texas Instruments
Texas Instruments
TI-84 Silver Edition
TI-84 Silver Edition
TI-84 Silver Edition
CALIBRATION SENSOR
MONITOR VOLTAGE, WHEN
STABLE,PRESS [ENTER].
VALUE VOLTAGE
POINT 1:
POINT 2: 2
ENTER VALUE
? 7
CALIBRATION PH
CALIBRATION: LINEAR
SLOPE INT
1:OK
2:CALIBRATE NOW
3:MANUAL ENTRY
(5) Press 7 key then the ENTER key
(6) Press 1 twice to return to default menu screen
(4) Press ENTER key

23. End of Tutorial 4 Menu Options Back to Beginning Previous Slide
Finished