1. PhyNexus gDNA from tissue
06/12/13

2. Five different tissues tested
Live and dried cricket
Live and dried worm
Goldfish
Live worm was chosen for optimization:
Most gDNA generated
Easy to handle

3. Other variables tested
Tested different lysis conditions (different buffers as well as different temperatures)
Tested different methods with different buffer components

4. Current working method
20-25 mg of worm
Protease
Overnight Lysis at 56*C
2nd Lysis at 70*C
RNAse
Capture by gravity/pressure
Wash
Dry
Elute

5. Genomic DNA purified using PhyTip columns is compatible with PCR application
gDNA was purified using either (Q) Qiagen technology or
(P) PhyNexus technology. Following purification, these gDNA was used to amplify 18S ribosomal gene. Following PCR amplified DNA is loaded on a gel.
PhyNexus gDNA purification technology is compatible with PCR application
18S Ribosome (highly conserved from organism to oranism

6. Further work
We are currently satisfied with yield and performance of the technology
Test different lysing conditions including room temperature second lysing