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Published byIrma Norton Modified over 6 years ago
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Cocci
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Bacilli
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Spirochetes
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Staphylococcus Arrangement
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Diplococcus
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Streptococcus
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Streptobacillus Arrangement
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raised, Whole Colony MARGIN ELEVATION round irregular filamentous
rhizoid MARGIN Smooth, entire rhizoid Irregular (erose) lobate filamentous ELEVATION convex umbonate plateau flat raised, spreading edge flat raised margin growth into medium raised
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Opaque Center and Spreading Edge
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Shiny and Umbonate
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Irregular and Rhizoid
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Punctiform Colonies
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Stains
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Wet Mount (Hanging Drop)
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Cheek Cells Without Stain
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Cheek Cells with Simple Stain (Methylene Blue)
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Simple Stain: Negative Stain
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Negative Stain
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Differential Stain: Gram Stain
Color of Gram + Gram – Primary stain: Crystal violet Purple Mordant: Iodine Decolorizing agent: Alcohol-acetone Colorless Counterstain: Safranin Pink
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Outer membrane Peptidoglycan GRAM NEGATIVE GRAM POSITIVE
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Gram Positive and Gram Negative Bacteria
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Gram Stain of Staphylococcus epidermidis (+) and Citrobacter diversus (-).
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Acid-Fast Stain ZN Method
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Acid-Fast and Non Acid-Fast
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Endospore Stains
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Endospores
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Endospore Stain: Ziehl-Neelsen
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Figure 3.12a-c
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Endospore Stain
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Stain Comparisons G+ (PURPLE) G – (PINK) ACID FAST PRIMARY STAIN
ENDOSPORE STAIN PRIMARY STAIN Crystal violet Carbol Fuschia Malachite green MORDANT Iodine Heat DECOLORIZER ETOH or acetone: Cell is purple Cell is clear Acid alcohol Water COUNTER-STAIN Safranin: Cell is pink Methylene Blue Spores are
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Capsule Stain
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Capsule Stain
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Figure 3.12a-c
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Flagella Stain
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Flagella Stain
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Peritrichous Flagella
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Figure 3.12a-c
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Names for flagella arrangement
Monotrichous Lophotrichous Amphitrichous Peritrichous
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