1. Fluid membranes From biology to physics and back again
Introduction 1
Fluid membranes From biology to physics and back again
TEM image
Ana-Sunčana Smith
Institut für Theoretische Physik I
Universität Erlangen-Nürnberg, Germany

2. Statistical mechanics at reduced dimensionality
Introduction 2
Statistical mechanics at reduced dimensionality
self assembly – structural properties
phase transitions (solid-gel-fluid)
microfluidics
mixtures - lateral diffusion, aggregation
domain formation
thermal fluctuations
Soft matter
interplay of the bending elasticity with the
surface tension and adhesion
chemical kinetics in confined geometry
interplay between length and time scales
Biomimetics
understanding the relation between the physical
properties of soft materials and their biological role
Nano/Bio-technology
bio-compatible materials and systems
micro-manipulators and molecular probes
bio-sensors
Cluster of Excellence: Engineering of Advanced Materials

3. Chemical composition PtdCho – Phosphatidylcholine
PtdEtn - Phosphatidylethanolamine
PtdIns - Phosphatidylinositol
PtdSer - Phosphatidylserine

4. Chemical composition
Phosphoglycerides

5. Chemical composition
Sphingomyelin
Phosphoglycerides

6. Chemical composition Glycolipid- cerrebroside 13.11.2018
Cluster of Excellence: Engineering of Advanced Materials

7. Chemical composition
Cluster of Excellence: Engineering of Advanced Materials

8. Chemical composition
Cluster of Excellence: Engineering of Advanced Materials

9. Chemical composition Cholesterol
Space association of SPHM and cholesterol
Cluster of Excellence: Engineering of Advanced Materials

10. Self assembly

11. Self assembly
Phase diagram for monoolein

12. Mixtures

13. Self assembly
Self assembly
Degrees of freedom

14. Pipetting vesicles

15. The cell membrane

16. Cell membranes

17. Cellm membranes
Cluster of Excellence: Engineering of Advanced Materials

18. Principle curvatures

19. Basic membrane shapes Shape C1 C2 Plane Cylinder + Sphere Saddle -
Cylinder +
Sphere
Saddle -
a | A membrane under a flat clathrin lattice.
b | A membrane tube under a dynamin coat.
c | The neck of a membrane bud.
d | Pure clathrin cages assembled in vitro.
(Heuser Lab for more fascinating images).
Zimmerberg J and Kozlov MM (2005)
Nature Reviews Molecular Cell Biology 6: 1

20. Spontaneous curvature
Mechanisms by which proteins can generate membrane curvature.
a | The scaffold mechanism. A rigid protein, or protein domain (for example, the BAR (Bin, amphiphysin, Rvs) domain), that has an intrinsic curvature binds to the membrane surface and bends the membrane beneath it.
b | Polymerized coat proteins, which are sometimes linked to membranes through adaptor proteins (not shown), stabilize membrane curvature.
c | The local spontaneous curvature mechanism is mediated by the insertion of amphipathic moieties of proteins between the polar headgroups of lipid molecules.
Zimmerberg J and Kozlov MM (2005) Nature Reviews Molecular Cell Biology 6: 1

21. Reduced volume
p < p Dp

22. Vesicle morphology
The area difference model

23. Area difference for the RBC

24. Fluctuation amplitude

25. Measurement of membrane fluctuations
Betz and Sykes Soft Matter, 2012,8,