1. Hydrocortisone reduced in vivo, inflammation-induced slow rolling of leukocytes and their extravasation into human conjunctiva
by Juha Kirveskari, Maaret Helintö, Jukka A. O. Moilanen, Timo Paavonen, Timo M. T. Tervo, and Risto Renkonen
Blood
Volume 100(6):
September 15, 2002
©2002 by American Society of Hematology

2. Video analysis of leukocytes at the site of inflammation
Video analysis of leukocytes at the site of inflammation.PRE indicates analysis performed before surgery, and INF indicates analysis 24 hours after surgery when the inflammatory reaction was most vivid.
Video analysis of leukocytes at the site of inflammation.PRE indicates analysis performed before surgery, and INF indicates analysis 24 hours after surgery when the inflammatory reaction was most vivid. Patients without hydrocortisone treatment are marked with blue bars and patients with hydrocortisone treatment with black. Pvalues between the groups were calculated with Mann-WhitneyU test. *P <.05. (A) Number of rolling cells in conjunctival venules was significantly higher in specimens taken during inflammation than in specimens taken before inflammation (groups pooled in the Figure), and hydrocortisone had no effect on this variable. (B) Relative frequencies of leukocyte rolling velocities in specimens taken during the inflammation, with or without preoperative hydrocortisone treatment. Leukocyte rolling in the nontreated patients was significantly slower than in the hydrocortisone-treated patients. (C) Number of extravasated leukocytes per square millimeter in conjunctival tissue. Specimens taken before the inflammation contained essentially no leukocytes, but the number was significantly elevated in the specimens taken during the inflammation versus those taken before the inflammation, and hydrocortisone treatment significantly reduced their numbers.
Juha Kirveskari et al. Blood 2002;100:
©2002 by American Society of Hematology

3. Comparison of digital noninvasive video “biopsies” with classical invasive histological biopsy.(A) Video image of conjunctival capillary from a specimen taken before the inflammation showing no rolling leukocytes near the inner surface of the vessel lined b...
Comparison of digital noninvasive video “biopsies” with classical invasive histological biopsy.(A) Video image of conjunctival capillary from a specimen taken before the inflammation showing no rolling leukocytes near the inner surface of the vessel lined by endothelium and no leukocytes within the conjunctival stroma. (B) Strong tissue inflammation induced by local cataract surgery is visible just above the capillary diving into the surface pictured. Another hot spot of tissue-invading cells is visible at the upper right (white arrows). (C) Specimen taken during the inflammation with hydrocortisone pretreatment: capillary with several rolling leukocytes (white arrows) tethering to vascular endothelium. Several cells are visible within the tissue near the vessels. (D) A classical histological biopsy from the same patient as in panel B. Several tissue-infiltrating leukocytes stained with anti sLex antibodies (brown) are visible within the tissue; 3 intravascular leukocytes and red blood cells (blue) are depicted within a vessel cross-section marked with white dotted lines. A 1-minute video (Cataract version 2 video; size, 12 Mb; format, quick time movie) demonstrating the leukocyte rolling during inflammation in capillaries, extravasated cells within the tissue, as well as the effects of hydrocortisone on these parameters is available via the University of Helsinki Web site ( Original magnification A-C, × 200; D, × 400.
Juha Kirveskari et al. Blood 2002;100:
©2002 by American Society of Hematology

4. Analyses of endothelial expression of P-selectin and sulfated sLex glycans on L-selectin ligands in conjunctiva.The percentage of conjunctival venules expressing P-selectin (A); MECA-79, reactive with sulfated extended core 1 mucin–type O-glycans (E); 2F3 d...
Analyses of endothelial expression of P-selectin and sulfated sLex glycans on L-selectin ligands in conjunctiva.The percentage of conjunctival venules expressing P-selectin (A); MECA-79, reactive with sulfated extended core 1 mucin–type O-glycans (E); 2F3 detecting sLex epitopes in the specimens taken before and during the inflammation without (blue bars) and with (black bars) hydrocortisone pretreatment (I). P values between the groups were calculated with Mann-Whitney U test. *P < .05. (B) (F) (J) Isotype-matched background controls visible with essentially no reactivity. (C) (G) (K) Staining of specimens taken before the inflammation where a small proportion of vessels react with anti–P-selectin antibodies (brown in panel C), but no MECA-79 reactivity is recorded. The 2F3 stains epithelial sLex, but the endothelium lining the vessels is negative. (D) (H) (L) Staining of specimens taken during the inflammation for endothelial P-selectin, MECA-79, and 2F3 with reactive endothelium within vessels (red-brown) is visible in all panels. Original magnification, × 200.
Juha Kirveskari et al. Blood 2002;100:
©2002 by American Society of Hematology