1. HS 4160 Critical Scientific Analysis
Week 4: Utility of peripheral blood dual color, double fusion fluorescent in situ hybridization for BCR/ABL fusion to assess cytogenetic remission status in chronic myeloid leukemia
HS 4160 Critical Scientific Analysis

2. According to the authors, D-FISH stands for:
De-stained FISH
Dual color FISH
Dual color, double fusion FISH
Tri color, double fusion FISH

3. One limitation of D-FISH is failure to detect cryptic translocation
True
False

4. D-FISH could Significantly increase false positive events
Significantly decrease false negative events
Significantly decrease sensitivity
Significantly decrease specificity

5. The only exclusion criteria the authors used was:
Prior history of other cancers
Prior history of AML
Prior history of any CML-directed therapy
Prior history of conventional cytogenetic testing

6. Which of the following correctly describe the probe set used?
ABL gene labelled with green
BCR gene on 9q34
This is a home-made probe set
Fusion indicates ABL/BCR translocation

7. Which software program the authors used for their statistical analysis?
SPSS
SAS
Excel
GraphPad

8. From table I, which triplicate collection showed best correlation?

9. The outlier from Figure 1 had:
no BCR/ABL fusion in bone marrow but with t(9;22) by CC
no t(9;22) by CC but high rate of BCR/ABL fusion in bone marrow
no t(9;22) by CC but high rate of BCR/ABL fusion in blood
no BCR/ABL fusion in blood but with t(9;22) by CC

10. From table II, which pair has more samples?
Bone marrow D-FISH vs. CC
Peripheral blood D-FISH vs. CC
Peripheral blood D-FISH vs. Bone marrow D-FISH