1. GAL 3
GALACTOSE +
ATP
GAL 3
GALACTOSE
ATP
GAL 80
GAL 4

2. Specific induction factors are, exclusively and typically in fungi, Zn binuclear cluster proteins.

3. A/SC-2-C-6-C-4/7-P-0/1-C-2-C-4/7-C

5. UaY: CGG-6x-CCG
PPR1: CGG-6x-CCG
GAL4: CGG-11x-CCG
PUT3: CGG-10x-CCG

6. 5’ CGG-X-CCG Inverted
GCC-X-GGC 5’
5’ CGG-X-CGG Direct
GCC-X-GCC 5’
5’ CCG-X-CGG Everted
GGC-X-GCC 5’

9. Protein: CreA Zn finger
competitor
wt prnd prnd wt
wt probe prnd22 probe

10. +
Treatment
Denature

11. FP C

15. Protein: CreA Zn finger
competitor
wt prnd prnd wt
wt probe prnd22 probe

16. +
RETARDATION GEL FP C

17. +
FP C
RETARDATION GEL FP C

18. FP C

19. +
FP C
RETARDATION GEL C

20. FP C

21. Treatment (DMS)
DNA extraction
Piperidine
Specificprimer extension
Ligation
(non-specific linker)
PCR
Thus, generation of molecules within the region of interest, all ending at a methylation site
Primer extension
Thus, generation of molecules, terminally labelled on a single strand, all starting at the same
point, all ending at methylation site, continuation as for any foot print.

23. Non-induced Induced
GAL1
GAL10
UAS
PHO5

25.