1. IRES‐dependent translation is specifically abolished by an IRES point mutation.
IRES‐dependent translation is specifically abolished by an IRES point mutation. (A) Immunoblotting for the β‐globin protein. The upper band corresponds to the extended β‐globin product from the WT+300+e3 transfection control and the lower band corresponds to β‐globin protein produced from the cap WT construct (lane 1), the mut IRES WT construct (lane 2), the IRES WT construct (lane 3) or empty vector (lane 4). (B) Luminometer measurements of firefly luciferase produced from the bicistronic luciferase constructs shown in Fig 1D,E, after normalization to Renilla luciferase activity. Ctrl, control; IRES, internal ribosome entry sequence; mut, mutant; WT, wild type.
Jill A Holbrook et al. EMBO Rep. 2006;7:
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