1. Volume 149, Issue 2, Pages 341-349 (May 2018)
Helicase POLQ-like (HELQ) as a novel indicator of platinum-based chemoresistance for epithelial ovarian cancer 
Jing Long, Jun-You Zhu, Yong-Bin Liu, Kun Fu, Yan Tian, Pei-Yao Li, Wen-Qing Yang, Si-Yu Yang, Ji-Ye Yin, Gang Yin, Yu Zhang 
Gynecologic Oncology 
Volume 149, Issue 2, Pages (May 2018)
DOI: /j.ygyno
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2. Gynecologic Oncology 2018 149, 341-349DOI: (10.1016/j.ygyno.2018.03.006)
Copyright © 2018 The Authors Terms and Conditions

3. Fig. 1 HELQ and patient response to therapy and prognosis.
(A) and (B) Kaplan-Meier survival curves for OS A) (n=485) and DFS B) (n=396) for EOC patients with HELQ low and high expression from The Cancer Genome Atlas (TCGA) datasets.
(C) and (D) Kaplan-Meier survival curves for OS C) (n=87) and DFS D) (n=66) for EOC patients with HELQ low and high expression in our study, ovarian cancer case patients were stratified into HELQ low and high expression according to mean of HELQ expression.
OS=overall survival; DFS=disease-free survival.
Gynecologic Oncology  , DOI: ( /j.ygyno )
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4. Fig. 2
HELQ expression in human EOC tissues of platinum-sensitive and platinum-resistant patients.
(A) Representative immunohistochemical staining of HELQ in EOC (−, +, ++).
(B) Percentage distribution of different immunohistochemical staining of HELQ expression in ovarian cancer specimens of chemo-sensitive and chemo-resistant.
(C) and (D) HELQ mRNA and HELQ protein expression levels in ovarian cancer specimens of chemo-sensitive and chemo-resistant analyzed by C) RT-PCR and D) Western blotting, respectively.
Gynecologic Oncology  , DOI: ( /j.ygyno )
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5. Fig. 3 Effect of HELQ on cellular response to cisplatin.
(A) A2780, OVCAR3 cellular response to cisplatin, both cells were treated with various concentrations of cisplatin for 48h and followed by CCK8 assay;
(B) and (C) HELQ mRNA and protein expression level in A2780, OVCAR3 cells.
(D) A2780-CP70 were more resistant to cisplatin compared with its parental A2780 cells;
(E) and (F) HELQ mRNA and protein expression level were increased in A2780-CP70 cells.
(G) OVCAR3 cells with HELQ overexpression (O3-HELQ) were more resistant to cisplatin compared to the cells transfected with vector lentivirus control (O3-CTR);
(J) A2780 cells with HELQ knocked down (A2-siHELQ) were more sensitive to cisplatin compared to the cells transfected with scramble control (A2-siCTR);
(H), (K) and (I), (L) All proteins and mRNA were detected using Western blot and RT-PCR. The data were from at least 3 independent experiments.
Gynecologic Oncology  , DOI: ( /j.ygyno )
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6. Fig. 4
Repair of UV damage in luciferase reporter plasmid under different HELQ expression level.
(A) and (B) OVCAR3 stable cell line with HELQ overexpression OVCAR3-HELQ and its lentivirus control OVCAR3-CTR, A2780 stable cell line with HELQ knockdown A2780-siHELQ and its lentivirus control A2780-siCTR transiently transfected with a firefly luciferase reporter plasmids irradiated with different times of 10J UV followed by analysis of luciferase activity. A plasmid encoding Renilla luciferase was cotransfected for normalization of transfection efficiency.
DRC was calculated as the percentage of luciferase activity from irradiated reporter to that of the unirradiated reporter.
(C) PCR analysis of UV damaged plasmids. Lane 1: unirradiated intact plasmid control; lanes 2–4: plasmids irradiated by 10J UV with 1, 2, 4, 8min.
Gynecologic Oncology  , DOI: ( /j.ygyno )
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7. Fig. 5 Effect of HELQ expression on NER protein.
OVCAR3-HELQ and OVCAR3-CTR stable cell line, A2780-siHELQ and A2780-siCTR stable cell line were subjected to Western blot analyses of HELQ, ERCC1, XPC, XPA, RPA32, RPA70. GAPDH was used as a loading control for Western blot.
Gynecologic Oncology  , DOI: ( /j.ygyno )
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8. Fig. S1
The level of HELQ increased with the prolongation of cisplatin treatment time A2780 cells treated with 10μmol/L cisplatin at different points within 48h.
Gynecologic Oncology  , DOI: ( /j.ygyno )
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9. Fig. S2
Effect of HELQ expression on protein of ATM pathway OVCAR3‐CTR and OVCAR3‐HELQ stable cell line were subjected to Western blot analyses of GAPDH, HELQ, ATM, c‐Abl. GAPDH was used as a loading control for Western blot.
Gynecologic Oncology  , DOI: ( /j.ygyno )
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