1. WS 3 Monitoring extent and patterns of use of NPS
Identification and assessment of new psychoactive substances: a European network HOME/2014/JDRU/AG/DRUG/7086
WS 3
Monitoring extent and patterns of use of NPS
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2. 4 Partners + 2 Associate Partners
Istituto di Ricerche Farmacologiche Mario Negri (IRFMN), Italy
Universitat Jaume I de Castellon (UJI), Spain
Facultade de Farmacia, Universitade de Lisboa (FFULIS), Portugal
Istituto Nacional de Medicina Legal e Ciencias Forenses (INMLFC), Portugal
Norsk Institutt for vannforskning (NIVA), Norway
Egas Monitz - Cooperativa de Ensino Superior (EMCES), Portugal
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3. 3 pillars of the project Identification Assessment NPS
“Quantification”
Title: Monitoring extent and patterns of use of NPS (Monitoring the most widely-used NPS in European cities)
Activities: The NPS identified and selected in WS1 and WS2 will be monitored in wastewater and urine
Aim: Assess NPS extent and pattern of use in the population by combining wastewater analysis and urine analysis in cities in Europe
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4. Gantt - time table Months 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
Months
Activities 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
WS0
WS1 - activity 1
WS1 - activity 2a
WS1 - activity 2b
WS1 - activity 3a
WS1 - activity 3b
WS1 - activity 3c
WS2
WS3 - activity 1
WS3 - activity 2
WS3 - activity 3
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5. WS 3 : Monitoring extent and pattern of use of NPS
Activity 1: synthesis of standard and metabolites (UJI, FFULISBOA)
- Purchase of reference standard
Reference standards already available (in IRFMN, …others ?)
Reference standards commercially available
- Synthesis of standard, metabolites and deuterated analogues (identified and refined in WS1 and WS2)
- Description of analytical parameters (retention time, isotopic distribution, fragment ions, fragmentation)
Aim: to get reference standards and analytical parameters to set up analytical methods (activity 2)
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6. WS3
Activity 2: analytical methods in wastewater (IRFMN, UJI, FFULISBOA, INMLCF, EMCES, NIVA)
Development, validation and standardization of analytical methods for NPS markers in wastewater
Inter-laboratory standardization ?
14/11/2018

7. WS3
Activity 3. analysis of NPS markers in wastewater (IRFMN, UJI, NIVA…….)
Analysis for markers in wastewater from EU cities (20 EU cities are foreseen or 2017 European SCORE study ? Ask for permission to the partners ?)
and music festivals (urine and wastewater in 2017 ? )
Repeated sampling in some cities (Milan, Lisbon…..)
Enantiomeric profiling studies
Activity 4: estimating NPS use in Europe (IRFMN, UJI, FFULISBOA, INMLCF, EMCES, NIVA)
Estimation of NPS use, trends and pattern of consumption (from analysis of markers in wastewater and in urine)
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8. Expected results
Identification, assessment and quantification of some of the NPS most widely used in Europe
Outputs:
A collection of about 100 substances (Standards, metabolites...)
2 Research papers
2 book chapters
Meeting/Postgraduate Course (2 events)
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