1. 6 A Tour of the Cell Lecture Presentation by Nicole Tunbridge and6
A Tour of the Cell
Lecture Presentation by Nicole Tunbridge and
Kathleen Fitzpatrick

2. The Fundamental Units of Life
All organisms are made of cells
The cell is the simplest collection of matter that can be alive
Cells are usually too small to be seen by the naked eye
Microscopes are used to visualize cells

3. Concept 6.2: Eukaryotic cells have internal membranes that compartmentalize their functions
The basic structural and functional unit of every organism is one of two types of cells: prokaryotic or eukaryotic
Only organisms of the domains Bacteria and Archaea consist of prokaryotic cells
Protists, fungi, animals, and plants all consist of eukaryotic cells

4. Prokaryotic cells are characterized by having
No nucleus
DNA in an unbound region called the nucleoid
No membrane-bound organelles
Cytoplasm bound by the plasma membrane

5. the bacterium Bacillus coagulans (TEM)
Figure 6.5
Fimbriae
Nucleoid
Ribosomes
Plasma membrane
Cell wall
Bacterial
chromosome
Capsule
0.5 µm
Flagella
Figure 6.5 A prokaryotic cell
(a)
A typical
rod-shaped
bacterium
(b)
A thin section through
the bacterium Bacillus
coagulans (TEM)

6. Eukaryotic cells are characterized by having
DNA in a nucleus that is bounded by a membranous nuclear envelope
Membrane-bound organelles
Cytoplasm in the region between the plasma membrane and nucleus
Eukaryotic cells are generally much larger than prokaryotic cells

7. The Nucleus: Information Central
The nucleus contains most of the cell’s genes and is usually the most conspicuous organelle
The nuclear envelope encloses the nucleus, separating it from the cytoplasm
The nuclear membrane is a double membrane; each membrane consists of a lipid bilayer

8. Concept 6.4: The endomembrane system regulates protein traffic and performs metabolic functions in the cell
The endomembrane system consists of
Nuclear envelope
Endoplasmic reticulum
Golgi apparatus
Lysosomes
Vacuoles
Mitochondria/cytoplasm
Plasma membrane

9. The Endoplasmic Reticulum: Biosynthetic Factory
The endoplasmic reticulum (ER):
Synthesizes lipids
Metabolizes carbohydrates
Detoxifies drugs and poisons
Stores calcium ions

10. The Golgi Apparatus: Shipping and Receiving Center
The Golgi apparatus consists of flattened membranous sacs called cisternae
Functions of the Golgi apparatus
Modifies products of the ER
Manufactures certain macromolecules
Sorts and packages materials into transport vesicles

11. Golgi apparatus cis face (“receiving” side of Golgi apparatus) 0.1 μm
Figure 6.12
Golgi
apparatus
cis face
(“receiving” side of
Golgi apparatus)
0.1 μm
Cisternae
Figure 6.12 The Golgi apparatus
trans face
(“shipping” side of
Golgi apparatus)
TEM of Golgi apparatus

12. Lysosomes: Digestive Compartments
A lysosome is a membranous sac of hydrolytic enzymes that can digest macromolecules
Lysosomal enzymes work best in the acidic environment inside the lysosome
Hydrolytic enzymes and lysosomal membranes are made by rough ER and then transferred to the Golgi apparatus for further processing

13. Some types of cell can engulf another cell by phagocytosis; this forms a food vacuole
A lysosome fuses with the food vacuole and digests the molecules
Lysosomes also use enzymes to recycle the cell’s own organelles and macromolecules, a process called autophagy

14. Vacuoles: Diverse Maintenance Compartments
Vacuoles are large vesicles derived from the ER and Golgi apparatus
Vacuoles perform a variety of functions in different kinds of cells:
hold organic compounds and water
In protists, pump excess water out of cells

15. Nucleus Rough ER Smooth ER cis Golgi Plasma membrane trans Golgi
Figure 6.15
Nucleus
Rough ER
Smooth ER
cis Golgi
Figure 6.15 Review: relationships among organelles of the endomembrane system
Plasma
membrane
trans Golgi

16. Concept 6.5: Mitochondria and chloroplasts change energy from one form to another
Mitochondria are the sites of cellular respiration, a metabolic process that uses oxygen to generate ATP
Chloroplasts, found in plants and algae, are the sites of photosynthesis

17. Mitochondria: Chemical Energy Conversion
Mitochondria are in nearly all eukaryotic cells
They have a smooth outer membrane and an inner membrane folded into cristae
Cristae present a large surface area for enzymes that synthesize ATP

18. Outer membrane Inner membrane
Figure 6.17
Mitochondrion
10 μm
Intermembrane
space
Mitochondria
Outer
membrane
DNA
Free
ribosomes
in the
mitochondrial
matrix
Inner
membrane
Mitochondrial
DNA
Cristae
Nuclear DNA
Matrix
Figure 6.17 The mitochondrion, site of cellular respiration
0.1 μm
(a)
Diagram and TEM of mitochondrion
(b)
Network of mitochondria in
Euglena (LM)

19. Chloroplasts: Capture of Light Energy
Chloroplasts contain the green pigment chlorophyll, as well as enzymes and other molecules that function in photosynthesis
Chloroplasts are found in leaves and other green organs of plants and in algae

20. Inner and outer membranes
Figure 6.18
Chloroplast
Ribosomes
Stroma
50 μm
Inner
and outer
membranes
Granum
Chloroplasts
(red)
Figure 6.18 The chloroplast, site of photosynthesis
DNA
Thylakoid
Intermembrane space
1 μm
(a)
Diagram and TEM of chloroplast
(b)
(b)
Chloroplasts in an
algal cell

21. Concept 6.6: The cytoskeleton: Support and Motility
The cytoskeleton is a network of fibers extending throughout the cytoplasm
The cytoskeleton helps to support the cell and maintain its shape
Guiding movement of organelles
It is made up of Microtubules
In animal cells, microtubules grow out from a centrosome near the nucleus

22. Centrosome Microtubule Centrioles 0.25 μm Longitudinal section of one
Figure 6.22
Centrosome
Microtubule
Centrioles
0.25 μm
Figure 6.22 Centrosome containing a pair of centrioles
Longitudinal
section of one
centriole
Cross section
of the other centriole
Microtubules

23. Cilia and flagella share a common structure
Cilia and Flagella: microtubule-containing extensions that project from some cells
Cilia and flagella share a common structure
A core of microtubules covered by the plasma membrane
A basal body that anchors the cilium or flagellum
A motor protein called dynein, which drives the bending movements of a cilium or flagellum

24. Animation: Cilia and Flagella

25. Video: Microtubule Sliding in Flagellum Movement

26. Cell Walls of Plants
The cell wall is an extracellular structure that distinguishes plant cells from animal cells
Prokaryotes, fungi, and some unicellular eukaryotes also have cell walls
The cell wall protects the plant cell, maintains its shape, and prevents excessive uptake of water

27. Cell Junctions
Neighboring cells in tissues, organs, or organ systems often adhere, interact, and communicate through direct physical contact.
Three types of cell junctions are common in epithelial tissues
At tight junctions, membranes of neighboring cells are pressed together, preventing leakage of extracellular fluid. Found in Bladder, kidney, intestines

28. 2. Desmosomes (anchoring junctions) fasten cells together into strong sheets. Found in tissues which experience stress (provides extra space for relieving stress) Found in intestine, skin.
3. Gap junctions (communicating junctions) provide cytoplasmic channels between adjacent cells. Acts like a tunnel between two cells. Found in cells which provide action potential; e.g Heart cells, Nervous tissue cells and in placenta to send out strong contractions during birth.

29. Tight junctions prevent fluid from moving across a layer of cells.
Figure 6.30
Tight junctions prevent
fluid from moving
across a layer of cells.
Tight
junction
TEM
0.5 μm
Tight junction
Intermediate
filaments
Desmosome
Desmosome
(TEM)
1 μm
Gap
junction
Figure 6.30 Exploring cell junctions in animal tissues
Ions or small
molecules
Plasma
membranes of
adjacent cells
Extracellular
matrix
TEM
Space
between cells
0.1 μm
Gap junctions

30. Figure 6.UN04
Figure 6.UN04 Summary of key concepts: endomembrane system

31. Figure 6.UN05
Figure 6.UN05 Summary of key concepts: mitochondria, chloroplasts and peroxisomes