2. DNA strand synthesis by formation of phosphodiester bonds.
The chain is terminated by the use of dideoxycytidine triphosphate (ddC)
in place of deoxycytidine triphosphate (dCTP).

3. Cycle sequencing

4. One cycle of dye terminator cycle sequencing

5. one-color/four-lane method
such as
radioactive sequencing
Four-color/one-lane
fluorescent sequencing

7. blue - green -yellow - red
Spectral overlap of the dyes
in the four virtual filters
(vertical gray bars).
blue - green -yellow - red
540 nm
570 nm
595 nm
625 nm
Example of a matrix

8. Sequence data obtained from a plasmid with BigDye terminators.