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Optimising direct PCR from anagen hair samples

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1 Optimising direct PCR from anagen hair samples
Renée Ottens, Duncan Taylor, Damien Abarno, Adrian Linacre  Forensic Science International: Genetics Supplement Series  Volume 4, Issue 1, Pages e109-e110 (January 2013) DOI: /j.fsigss Copyright © 2013 Elsevier Ireland Ltd Terms and Conditions

2 Fig. 1 Chromatogram of nuclear DNA from a single anagen hair root, amplified using AmpFλSTR® NGM™ kit at 29 cycles on a GeneAmp® System 9600 thermal cycler. Figure shows 8 loci from NGM™ kit. PCR sample was analysed neat and diluted to a concentration of 1:50 before it was injected on an Applied Biosystems 3130xl Genetic Analyser at 3kV for 10s. Red circles indicate split peaks and black squares indicate pull-up in the neat sample. Split peaks and pull-up is not observed in the diluted sample. Forensic Science International: Genetics Supplement Series 2013 4, e109-e110DOI: ( /j.fsigss ) Copyright © 2013 Elsevier Ireland Ltd Terms and Conditions


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