1. Key differences in protein expression and pathway activation between SCLCs and NSCLCs. A, for each cell line, protein lysates were collected and analyzed by RPPA after growth in 10% serum, 0% serum, and serum-stimulated conditions (0% serum for 24 hours, th...
Key differences in protein expression and pathway activation between SCLCs and NSCLCs. A, for each cell line, protein lysates were collected and analyzed by RPPA after growth in 10% serum, 0% serum, and serum-stimulated conditions (0% serum for 24 hours, then 10% serum for 30 minutes before harvest) to account for possible effects of medium on protein expression. Unsupervised hierarchical clustering separated SCLCs (pink) from NSCLCs (green) on the basis of their distinct expression of 193 total and phosphoproteins. Clustering was independent of growth conditions, with lysates from the same cell line (but different media conditions) clustering together as nearest neighbors. NSCLC cell lines with neuroendocrine features—H1155 [large cell (LC)] and H1770 [neuroendocrine (NE); blue]—clustered with SCLC cell lines based on similar protein expression patterns. B, first principal component analysis using all RPPA proteins also separated NSCLC cell lines from SCLC cell lines. C, protein markers most differentially expressed between SCLCs and NSCLCs based on a false discovery rate (FDR) <1% and ≥1.5-fold difference in mean expression. Cell lines are clustered by hierarchical clustering and results from all media conditions are shown. NSCLC cell lines with neuroendocrine features (LCNE, blue) clustered with SCLCs (orange) based on similar protein expression. D, proteins expressed at higher levels in SCLCs or NSCLCs are mapped to their respective signaling pathways.
Lauren Averett Byers et al. Cancer Discovery 2012;2:
©2012 by American Association for Cancer Research