1. Volume 67, Issue 1, Pages 33-41 (January 2015)
Exosomal miR-1290 and miR-375 as Prognostic Markers in Castration-resistant Prostate Cancer 
Xiaoyi Huang, Tiezheng Yuan, Meihua Liang, Meijun Du, Shu Xia, Rachel Dittmar, Dian Wang, William See, Brian A. Costello, Fernando Quevedo, Winston Tan, Debashis Nandy, Graham H. Bevan, Sherri Longenbach, Zhifu Sun, Yan Lu, Tao Wang, Stephen N. Thibodeau, Lisa Boardman, Manish Kohli, Liang Wang 
European Urology 
Volume 67, Issue 1, Pages (January 2015)
DOI: /j.eururo
Copyright © 2014 European Association of Urology Terms and Conditions

2. Fig. 1
RNA species and their distributions in plasma exosomes. Raw reads are the sequences detected by RNA sequencing. Query reads are those after trimming. Mappable reads are those mapped to known human RNA or genome.
lncRNA=long noncoding RNA; miRNA=micro RNA; piRNA=piwi-interacting RNA; rRNA=ribosomal RNA; snRNA=small nuclear RNA; snoRNA=small nucleolar RNA; tRNA=transfer RNA.
European Urology  , 33-41DOI: ( /j.eururo )
Copyright © 2014 European Association of Urology Terms and Conditions

3. Fig. 2
Evaluations of microRNA (miRNA) candidates for endogenous normalization. (A) NormFinder-determined intergroup and intragroup stabilities of 173 miRNA candidates; (B) intergroup and intragroup variations of six selected candidates; (C) normalized read counts of six selected candidates in 192 individuals.
European Urology  , 33-41DOI: ( /j.eururo )
Copyright © 2014 European Association of Urology Terms and Conditions

4. Fig. 3
MicroRNAs (miRNAs)-based survival analysis in follow-up cohort (time in months). (A, C) Kaplan-Meier curves show that relative expression levels of miR-375 and miR-1290 are associated with overall survival; (B) miR-1246 shows a trend association but not statistically significance; (D) combination of miR-1290 and miR-375 expressions demonstrated a strong synergistic effect.
European Urology  , 33-41DOI: ( /j.eururo )
Copyright © 2014 European Association of Urology Terms and Conditions

5. Fig. 4
Multivariate-based survival analysis in follow-up cohort. Kaplan-Meier curves show significant survival association in androgen-deprivation therapy (ADT) failure time defined as from (A) initiation of ADT for hormone-sensitive stage to development of castration-resistant prostate cancer; (B) prostate-specific antigen (PSA) level; (C) multivariate model. (D) Time-dependent area under the curve (AUC) analysis shows significant improvement of multivariate model over clinical factors-only model.
European Urology  , 33-41DOI: ( /j.eururo )
Copyright © 2014 European Association of Urology Terms and Conditions

6. Supplementary Fig. 1 – Kaplan-Meier plots for overall survival in screening cohort (n=23). Relative expression levels of miR-375, miR-1246, and miR-1290 are associated with overall survival.
European Urology  , 33-41DOI: ( /j.eururo )
Copyright © 2014 European Association of Urology Terms and Conditions

7. Supplementary Fig. 2 – Validation of normalization candidates using TaqMan quantitative real-time polymerase chain reaction method. With a pool of 10 primers in follow-up cohort, (a) expression variations and (b) stabilities of the five selected candidates are presented. When primer number of RT primer pool is reduced, the detected levels of the four candidates are increased (c) and their stabilities are significantly increased (d). All data represented in the scatter plots are sorted based on Ct value of miR-16-5p.
European Urology  , 33-41DOI: ( /j.eururo )
Copyright © 2014 European Association of Urology Terms and Conditions

8. Supplementary Fig. 3 – Validation of the normalization candidates using QIAGEN real-time qRT-PCR method. (a) Expression patterns and (b) stabilities of selected candidates re determined by QIAGEN qRT-PCR assay. All data represented in the scatter plots are sorted based on CT value of miR-16-5p.
European Urology  , 33-41DOI: ( /j.eururo )
Copyright © 2014 European Association of Urology Terms and Conditions

9. Supplementary Fig. 4 – Kaplan-Meier analysis of individual clinical factors. No survival association was found in (a) initial Gleason score, (b) age at the time of castration-resistant prostate cancer (CRPC) stage, (c) hemoglobin level at CRPC stage, and (d) the number of treatments after progression to CRPC stage.
European Urology  , 33-41DOI: ( /j.eururo )
Copyright © 2014 European Association of Urology Terms and Conditions