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Is hematopoietic stem cell homing deficient in Fanconi anemia?

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Presentation on theme: "Is hematopoietic stem cell homing deficient in Fanconi anemia?"— Presentation transcript:

1 Is hematopoietic stem cell homing deficient in Fanconi anemia?
by Lee O'Neill, Amy M. Skinner, and Peter Kurre Blood Volume 113(21): May 21, 2009 ©2009 by American Society of Hematology

2 CXCR4 expression and cell-matrix adhesion among hematopoietic progenitors from Fancc−/− mice and wild-type littermates. CXCR4 expression and cell-matrix adhesion among hematopoietic progenitors from Fancc−/− mice and wild-type littermates. (A) Cell-surface expression of CXCR4 on lineage-depleted (lin−) and whole bone marrow (WBM) cells from C57Bl/6 strain mice. Assay carried out as described.5 Briefly, cells were harvested from Fancc+/+ and Fancc−/− (not shown) animals, samples were depleted of red cells by hemolysis and lineage-depleted using an Easy Sep Mouse Hematopoietic Progenitor Cell Enrichment kit (StemCell Technologies, Vancouver, BC) according to the manufacturer's instructions. Samples were stained with antibodies (fluorescein isothiocyanate [FITC] label) directed against surface CXCR4 epitopes or IgG (FITC; all BD Biosciences, San Jose, CA). Expression was analyzed using a FACSCalibur (BD Biosciences) and data were processed using FlowJo software (TreeStar, Ashland, OR). (B) Cell-matrix adhesion by WBM progenitors from Fancc−/− and wild-type animals. The assay was performed using lineage-depleted progenitor cells generated as outlined above and was otherwise performed exactly as described by Zhang.1 Lee O'Neill et al. Blood 2009;113:5361 ©2009 by American Society of Hematology

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