1. Bacterial Siderophore Production: When, Where, and Why?
Donald Martocello
Geosciences Class of 2018

2. Growing Bacteria and Vibrio harveyi
Iron = essential trace nutrient.
Siderophores are molecules used to acquire iron.
Vibrio harveyi is a model bacterium.
To study we need a well-defined, clean medium.

3. Focusing on Nitrogen Nitrogen is crucial for cell growth.
Amino Acids -> Proteins -> Healthy Cultures
Experimented with different sources to find the most ideal:
Nitrate (NO3-)
Ammonium (NH4+)
Urea (CH4N2O)
Varying concentrations and specific amino acids given

4. Determining the Most Ideal Source of Added Nitrogen
Amino acids and ammonium are most successful.
Excluding L-Marine and Just SLIMV, total N present is 27.6 mM.

5. Which amino acids can they make?
Genomic research showed V. harveyi can make all amino acids.
Ammonium transfer experiments.
Growth rate = ~0.21 hr-1

6. Determining the Ideal Combination of Amino Acids
Our research suggested that both amino acid type and concentration are important factors in defining a successful medium.
 Increasing Proportion of Amino Acids to Ammonium

7. Amino Acid Usage Data
Comparing the amino acids added to how they are being used.

8. Future Applications in Trace Metal Research
Still more to refine.
With a clean medium, we can study siderophores and trace metals.
Bacterial iron quotas.
Exploration of other metal quotas.
Applications in other Vibrio species.

9. Acknowledgements
Special Thanks to Professor Morel, Darcy McRose, and the entire Morel Group!