1. Volume 56, Issue 1, Pages 123-133 (July 2009)
Mode-of-Action, Efficacy, and Safety of a Homologous Multi-Epitope Vaccine in a Murine Model for Adjuvant Treatment of Renal Cell Carcinoma 
Christian Doehn, Norbert Esser, Hans-Gerd Pauels, Stephan T. Kießig, Matthias Stelljes, Armin Grossmann, Dieter Jocham, Joachim Drevs 
European Urology 
Volume 56, Issue 1, Pages (July 2009)
DOI: /j.eururo
Copyright © 2008 European Association of Urology Terms and Conditions

2. Fig. 1
General experimental design showing the setup of vaccinated donor animals and recipient animals for a CD8-depletion experiment.
European Urology  , DOI: ( /j.eururo )
Copyright © 2008 European Association of Urology Terms and Conditions

3. Fig. 2
(A) Mean weights of animals treated with a RENCA vaccine (lysate of 5×106 RENCA cells) with IFN-γ incubation (group B) vs weights of animals from the control group (G). Data for groups A and C–F not shown. (B) Mean weights of animals treated with a RENCA vaccine (lysate of 5×106 RENCA cells) without IFN-γ incubation (group E) vs weights of animals from the control group (G). Data for groups A–D and F not shown.
European Urology  , DOI: ( /j.eururo )
Copyright © 2008 European Association of Urology Terms and Conditions

4. Fig. 3
(A,B) Mean tumour volumes of animals treated with different doses of RENCA vaccine (A) with IFN-γ incubation (groups A–C and B) without IFN-γ incubation (groups D–F) vs mean tumour volumes of animals from the control group (bars, SEM; day 21 after tumour transplantation). (C,D) Mean number of lung metastases of animals treated with different doses of RENCA vaccine (C) with IFN-γ incubation (groups A–C and D) without IFN-γ incubation (groups D–F) vs mean tumour volumes of animals from the control group (bars, SEM; day 21 after tumour transplantation).
***p<0.001 vs solvent control.
European Urology  , DOI: ( /j.eururo )
Copyright © 2008 European Association of Urology Terms and Conditions

5. Fig. 4
Ex vivo cytotoxicity of spleen cells from vaccine-treated and control mice against different target cells (day 21 after tumour transplantation). Cytotoxicity of spleen cells from BALB/c mice immunized with RENCA lysates corresponding to 106 (circles), 5×106 (squares), 107 (triangles) tumour cells or solvent only (open squares), was measured at different E:T ratios against three different target cells. All data points represent means±SD from N=12 animals per group (Unpaired t-test at E:T=100. ***p<0.001; **p<0.01; *p<0.05; n.s.: not significant).
European Urology  , DOI: ( /j.eururo )
Copyright © 2008 European Association of Urology Terms and Conditions

6. Fig. 5
Subcutaneous tumour volumes (mean±SD) following adoptive transfer of spleen cells and sera from RENCA-vaccinated BALB/c donor mice into naïve RENCA tumour-challenged recipient mice. (A) Transfer of nonfractionated spleen cells from RENCA-vaccinated mice (squares) and solvent control mice (diamonds). (B) Transfer of sera from RENCA-vaccinated mice (diamonds) and solvent control mice (squares). (C) Transfer of nondepleted spleen cells (squares) and CD8-depleted spleen cells (diamonds) from RENCA-vaccinated mice. (D) Transfer of nondepleted spleen cells (squares) and CD8-depleted spleen cells (diamonds) from RENCA-vaccinated mice.
European Urology  , DOI: ( /j.eururo )
Copyright © 2008 European Association of Urology Terms and Conditions