1. Lesson 054 Learning Goal: (You should be able to…)
Describe the affect of chromatography on ink
: (Can you …)
Make a chromatogram?
Obtain data from a chromatogram?

2. In your lab notebook lesson 054
Data in lab note book Chromatogram for brown ink

3. Vocabulary for solubility and chromatography
Solution
Solvent
Solute
Suspension
Colloid
Mixture
medium in chromatography
Dissolve
Solubility
Rate of solubility
Capillary action
Absorption/absorb

4. You need your Lab Notebook
Write To Think 054
You need your Lab Notebook
Read the AQUA sheet “Procedure for making a chromatogram”
Study the diagram about how to set up a chromatography tank and make a chromatogram. You have about 4 minutes.
Your teacher will then demonstrate how to make a chromatogram and explain why it’s useful and what you’ll use it for.
Put a title in your Lab notebook:
Chromatography

5. You need your Lab Notebook
Write To Think 054
You need your Lab Notebook
Read the AQUA sheet “Procedure for making a chromatogram”
Study the diagram about how to set up a chromatography tank and make a chromatogram. You have about 4 minutes.
Your teacher will then demonstrate how to make a chromatogram and explain why it’s useful and what you’ll use it for.
3. Put a title in your Lab notebook:
Chromatography

6. Plan of Action for today
How to set up a chromatography ‘tank’
Make a chromatogram (s)
Record qualitative and quantitative data
Debrief questions etc.

7. Chromatography
A method of separating the parts of a mixture using the different solubility of chemicals and the process of capillary action using a solvent (water) and a medium (paper towel).
It produces a colored (chrom) ‘picture’ (graph)
It is useful for identifying unknown chemicals by comparing the distance travelled and time taken with standard or baseline data of known chemicals.
FYI: Solubility is a physical property
FYI: Capillary action is a physical process

8. Instructions and demonstration
Backpacks, lab notebooks, etc.
Bench lay out … same at the end please
Equipment/materials … a set PER PERSON (scissors)
Take it all out (Paper towel … keep it clean!!)
Reliable, consistent, repeatable … because …
How to … demonstration and tips

9. Materials per person Large chromatography tank
Small water (solvent) reservoir/beaker
Stirring rod (medium support)
Paper clip
Paper towel (medium)
Stopwatch/timer
15 cm Ruler
Scissors

10. Chromatography tank set up
Stir rod
Paper clip
Paper towel
Pencil line
Brown marker spot
Water
See next slide before starting!

11. Collecting and Recording data
Time how long the ink takes to travel up the paper towel (medium) until you stop it by taking it out of the water (solvent). You decide when to take it out … but not too late!
Make a pencil mark CAREFULLY where the solvent reaches … do this IMMEDIATELY as the solvent will keep moving!
Measure the distance (cm) the INK travelled and solvent travelled from the original spot.
IN YOUR LAB NOTEBOOK
Title Chromatography
Record qualitative (what you see, description) and quantitative (what you measured, time, minutes, distance, cm) data
Scotch tape the chromatogram into your lab note book and label it. (It’s wet … so don’t tear it!)

12. Debrief Questions What happened to the brown ink? Why? How?
What does it imply? Infer?
What was the rate of movement? (Velocity!)
Do you need to change the water each time? Explain.

13. Design flaws and human mistakes
What are the possible human mistakes you could/did make? (What went ‘wrong’ or could go ‘wrong’?)
What are the design flaws with the chromatography set up?
What might affect the data?
What could affect the consistency of the data?
If you did this again with the SAME marker would you get the same data?
What must you do to make sure? How will you be consistent?

14. Variables and Constants
Possible Independent Variables, manipulated (IV): Dependent Variables, responding (DV): Constants, stays the same each time:

15. Summary 3 factors that might affect the rate of chromatography.
2 dependent variables that can be measured in chromatography.
1 reason chromatography is useful.

16. End of lesson

17. Plan of Action … PUT IN Lesson 055
Day 1: Monday 10/31: How to make a chromatogram. Make one or two. Collect data. (Quantitative and Qualitative.) What’s happening?
Day 2: Tuesday 11/01: Graffiti crime scene. Look at TPD data. Set up LNB and plan. Hypothesis. What to do if …
Day 3: Wednesday (34 min) 11/02: Collect data part 1 … short amount of time!
Day 4: Thursday (Mr. Thompson out) 11/03: Read about chromatography. Answer questions to help with understanding of how it works. Write hypothesis. What is solubility? What is capillary action?
Day 5: Friday 11/04: Collect data part 2
Day 6: Monday 11/07: Collect data part 3. Data organization and data analysis
Day 7: Tuesday 11/08: Data organization and data analysis.
Day 8: Wednesday (52 min … no late start) 11/09: Conclusion (CER) part 1
Day 9: Thursday 11/10: Conclusion (CER) part 2 MUST FINISH by end of DAY!
No school Friday 11th

18. Investigative question
How are different inks affected by chromatography?
Plan of Action for today:
How to set up a chromatography ‘tank’
Make a chromatogram and record data
Identify possible experimental flaws (and human mistakes)
Write an hypothesis

19. You’ll investigate …
Three colored inks
BLUE
YELLOW
BLACK