1. Simultaneous Genotyping of α-Thalassemia Deletional and Nondeletional Mutations by Real-Time PCR–Based Multicolor Melting Curve Analysis 
Qiuying Huang, Xudong Wang, Ning Tang, Tizhen Yan, Ping Chen, Qingge Li 
The Journal of Molecular Diagnostics 
Volume 19, Issue 4, Pages (July 2017)
DOI: /j.jmoldx
Copyright © 2017 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2. Figure 1
Location of the primers and probes used in the multicolor melting curve analysis assay. Schematic representation of the α-globin gene cluster, indicating the extent of the four deletions, position of the three nondeletional mutations, and relative positions of the primers and probes (except for the ABL-F and ABL-R internal positive control primers and probe P1, which are located on a different chromosome). Locations of X, Y, and Z sequence homology boxes and hypervariable regions are also shown.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2017 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3. Figure 2
Melting curve results from genomic DNA samples with various α-globin genotypes. Melting curves and corresponding genotypes of the four deletions and three nondeletional mutations and the wild-type (WT) allele are shown by their corresponding probes. Gray lines indicate the no template control. −dF/dT, negative derivative of fluorescence with respect to temperature versus temperature.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2017 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

4. Figure 3
Schematic illustration of the melting profiles of five genotypes that contained the HKαα allele (A) and the exact peak height ratios of HKαα/αα, HKαα/-α3.7, and -α3.7/αα in the multicolor melting curve analysis assay (B). Rm1 denotes the height of the -α3.7 allele melting peak and Rm2 the height of the wild-type (WT) allele melting peak, both of which were automatically obtained by the software of SLAN-96 real-time PCR system. Dotted lines indicate positions of the melting peaks. CS, Constant Spring; −dF/dT, negative derivative of fluorescence with respect to temperature versus temperature; QS, Quong Sze; WS, Westmead.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2017 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions