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Oxidative stress in follicular fluid of young women with low response compared with fertile oocyte donors Rocío Nuñez-Calonge, Susana Cortés, Luis Miguel Gutierrez Gonzalez, Roman Kireev, Elena Vara, Leonor Ortega, Pedro Caballero, Lisa Rancan, Jesús Tresguerres Reproductive BioMedicine Online Volume 32, Issue 4, Pages (April 2016) DOI: /j.rbmo Copyright © 2016 Reproductive Healthcare Ltd. Terms and Conditions
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Figure 1 Activity of glutathione peroxidase (GPx) (A), glutathione reductase (GR) (B) and glutathione S-transferase (GST) (C) (nmol/min/mg of proteins) in follicular fluid from donors and patients (low and high response). Data represent mean ± SEM. GPx and GR: *** P < low response patients compared with control and high response groups. GST: *** P < low response patients compared with control; ** P < high response patients compared with low response patients. One-way ANOVA analysis for GPx (F = 22.79, P < ), for GR (F = 58.92, P < ) and for GST (F = 30.82, P < ). Reproductive BioMedicine Online , DOI: ( /j.rbmo ) Copyright © 2016 Reproductive Healthcare Ltd. Terms and Conditions
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Figure 2 Malondialdehyde (MDA)+4-hydroxyalkenals content (µmol/mg of proteins) (A) and concentration of nitric oxide metabolites (NOx) (nmol/mg of proteins) (B) in follicular fluid from donors and patients (low and high response). Data represent mean ± SEM. MDA+4-hydroxyalkenals: ***P < low response patients compared with control and high response groups. NOx: *** P < low response patients compared with control; ** P < high response patients compared with control; Δ- P < high response patients compared with low response patients. One-way ANOVA analysis for MDA+4-hydroxyalkenals (F = 32.2, P < ) and for NOx (F = 76.84, P < ). Reproductive BioMedicine Online , DOI: ( /j.rbmo ) Copyright © 2016 Reproductive Healthcare Ltd. Terms and Conditions
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Figure 3 The concentrations of pro/anti-inflammatory cytokines IL-2 (A), IL-6 (B), IL-8 (C) and IL-10 (D) (pg/mg of proteins) in follicular fluid from donors and patients (low and high response). Data represent mean ± SEM. IL-2: *** P < low response patients compared with control; Δ- P < high response patients compared with low response patients. IL-6: *** P < low response patients compared with control; Δ- P < high response patients compared with low response patients. IL-8: *** P < low response patients compared with control. IL-10: *** P < low response patients compared with control and high response groups. One-way ANOVA analysis for IL-2 (F = 5.98, P = 0.015), IL-6 (F = 41.86, P < ), IL-8 (F = 8.76, P = ), IL-10 (F = 17.88, P < ). Reproductive BioMedicine Online , DOI: ( /j.rbmo ) Copyright © 2016 Reproductive Healthcare Ltd. Terms and Conditions
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Figure 4 The concentration of vascular endothelial growth factor (VEGF) in follicular fluid from donors and patients (low and high response). Data represent mean ± SEM. *** P < low response patients compared with control; ** P < high response patients compared with control. One-way ANOVA analysis for VEGF (F = 28.69, P < ). Reproductive BioMedicine Online , DOI: ( /j.rbmo ) Copyright © 2016 Reproductive Healthcare Ltd. Terms and Conditions
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