1. DNA Mutations and Repair
Chapter 17

2. Detecting mutations with the AMES test

4. SUMMARY OF AMES TEST

5. AMES TEST
Saccharin
Aflatoxin
2-aminofluorene

6. Aflatoxin B1

7. DNA Repair: general characteristics
Double stranded DNA
Redundancy of pathways

8. Types of DNA repair Mismatch repair Direct Repair Base-Excision Repair
Nucleotide Excision Repair

9. Mismatch repair
Mut H
Recognize GATC hemi-methylation
Mut S
Mut L

10. A closer look at mismatch repair

11. DIRECT REVERSAL REPAIR
Direct Repair

12. BASE EXCISION REPAIR

13. BASE EXCISION REPAIR

14. Nucleotide Excision Repair

15. Diseases of DNA repair!

16. Xeroderma pigmentosum
Autosomal recessive

17. Hereditary Non-Polyposis Colon Cancer
HNPCC
15% of all colon cancers

18. Li-Fraumeni
P-53 mutation