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Increased progenitor cell proliferation in the peripheral blood of patients with bronchial asthma: The role of nitric oxide  Chun-Hua Wang, MDa, Wen-Yih.

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Presentation on theme: "Increased progenitor cell proliferation in the peripheral blood of patients with bronchial asthma: The role of nitric oxide  Chun-Hua Wang, MDa, Wen-Yih."— Presentation transcript:

1 Increased progenitor cell proliferation in the peripheral blood of patients with bronchial asthma: The role of nitric oxide  Chun-Hua Wang, MDa, Wen-Yih Hsieh, MDa, Lee-Yung Shih, MDb, Horng-Chyuan Lin, MDa, Chien-Ying Liu, MDa, Kian Fan Chung, MDc, Han-Pin Kuo, MD, PhDa  Journal of Allergy and Clinical Immunology  Volume 104, Issue 4, Pages (October 1999) DOI: /S (99) Copyright © 1999 Mosby, Inc. Terms and Conditions

2 Fig. 1 Multiparameter flow cytometric analysis for enumeration of CD34+ progenitor cells. Data show staining with anti-CD45-FITC and anti-CD34-PE of blood NAMNC from patient with asthma. A, Sample stained with anti-CD45 FITC; region R1 represents nucleated leukocytes. Dashed line, Events of IgG-FITC. B, Anti- CD34-PE staining of cells in region R1. Region R2 represents CD34+ cells. Dashed line, Events of PE-conjugated control IgG. C, CD45 versus side-scatter analysis of events gated in region R1; region R3 identifies true CD34+ blast cells with low CD45 and low SCC characteristics. D, Light-scatter properties of cells gated by R1, R2, and R3. Region R4 represents blast/lymphocyte region into CD34+ cells scatter. Gate statistics of cells gated in R1 (G1), R1 plus R2 (G2), R1 to R3 (G3), and R1 to R4 (G4). Journal of Allergy and Clinical Immunology  , DOI: ( /S (99) ) Copyright © 1999 Mosby, Inc. Terms and Conditions

3 Fig. 2 Numbers of CD34+ progenitor cells in peripheral blood of healthy subjects (normal, n = 8) or patients with mild intermittent (mild, n = 12) or moderate persistent asthma (moderate, n = 8). Asterisk, P < .01 compared with healthy subjects; horizontal line, mean value. Journal of Allergy and Clinical Immunology  , DOI: ( /S (99) ) Copyright © 1999 Mosby, Inc. Terms and Conditions

4 Fig. 3 Mean fluorescence intensity (MFI) of iNOS activity of peripheral blood progenitor cells (open bars) or other NAMNCs (solid bars) in healthy subjects (normal, n = 8) or patients with mild intermittent (mild, n = 12) or moderate persistent (moderate, n = 8) asthma. Asterisk, P < .01 compared with progenitor cells in same group; pound sign, P < .01 compared with normal subjects. Data are means ± SEM. Journal of Allergy and Clinical Immunology  , DOI: ( /S (99) ) Copyright © 1999 Mosby, Inc. Terms and Conditions

5 Fig. 4 Colony numbers of progenitor cells cultured for 14 days in methylcellulose in presence (growth factor [+]) or absence of growth factors (growth factor [–]) from healthy subjects (normal, n = 7) or patients with mild intermittent asthma (asthma, n = 9). Asterisk, P < .01 compared with corresponding healthy subjects; pound sign, P < .01 compared with corresponding groups in absence of growth factors. Journal of Allergy and Clinical Immunology  , DOI: ( /S (99) ) Copyright © 1999 Mosby, Inc. Terms and Conditions

6 Fig. 5 Effect of L-NAME (1 mmol/L) or SNP on colony numbers of progenitor cells cultured for 14 days in methylcellulose in presence of growth factors, GM-CSF, SCF, and IL-3 from healthy subjects (normal, n = 7) or patients with mild intermittent asthma (asthma, n = 9). Asterisk, P < .01; pound sign, P < .05 compared with corresponding groups with growth factors alone. Journal of Allergy and Clinical Immunology  , DOI: ( /S (99) ) Copyright © 1999 Mosby, Inc. Terms and Conditions

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