1. Jim Collawn MCLM 350 jcollawn@uab.edu
GBS 740 Antigen Processing and Presentation Kuby, Immunology, 6th Edition Chapter 8
Jim Collawn
MCLM 350

2. Study Objectives
1. How do the peptide binding sites differ between MHC class I and II?
2. Compare and contrast class I and class II MHC-restricted
responses with regard to
A. source of antigens
B. antigen processing requirements (where are they degraded?)
C. role of chaperones (calnexin, calreticulin, ERp57, tapasin, HLA-DM)
D. types of T cell involved
Discuss the invariant chain’s role in the demarcation between class I and class II MHC-restricted responses.
What is cross-presentation?
What is MHC restriction?
6. Discuss HLA-DM’s possible role in antigen processing.

4. MHC class I
β2-microglobulin

5. MHC class II
DRβ chain

7. Model of the Solvent-accessible Area of class I H-2Kb
Vesicular
Stomatitis
Virus peptide
Sendia
virus
peptide

8. Antigen Processing and Presentation Where do the peptides come from and how do they become associated with the MHC molecules?

9. MHC Restriction

10. Antigen Processing Requirements for Helper T cell Activation

11. Cytosolic and Endocytic Pathways

12. Degradation of Intracellular Proteins

13. Transporter associated with Antigen Processing
Bare lymphocyte syndrome (BLS)

14. Generation of Peptide-class I MHC Complexes
ERAP1 (8 amino acids)

15. Assembly of MHC class I Molecules

18. Cross-
presentation
of exogenous
antigens
by MHC class I
molecules

19. Assembly of MHC class II Molecules

20. Study Objectives
1. How do the peptide binding sites differ between MHC class I and II?
2. Compare and contrast class I and class II MHC-restricted
responses with regard to
A. source of antigens
B. antigen processing requirements (where are they degraded?)
C. role of chaperones (calnexin, calreticulin, ERp57, tapasin, HLA-DM)
D. types of T cell involved
Discuss the invariant chain’s role in the demarcation between class I and class II MHC-restricted responses.
What is cross-presentation?
What is MHC restriction?
6. Discuss HLA-DM’s possible role in antigen processing.