2. Week 6 Team has split into Dry Lab and Wet Lab Dry Lab: Wet Lab:
Erika, - James,
Prudence, -Chris,
Clinton, -Tom,
Yanis, -Krupa,

3. Wet Lab
Two key tasks:
DNA synthesis
Protocols and Reagents

4. DNA Synthesis
Genes
Terminators
Promoter and RBS

5. Promoters RBS Promoter and RBS Inducible Constitutive 1.p43 Sigma B
1.Phyper-spank
2.pXyl
Constitutive 1.p43
2.pVeg
Sigma B
1.pctc
2.pgsiB
RBS
=12 combinations
1.gsiB
2.spoVG

6. Genes Genes Signal Peptide Biomaterials Registry Light+Motility 1.YtvA
2.EspE
Signal Peptide
1.SacB
2.LipA
Biomaterials
1.Elastin
2.EAK 16
Registry
1.Antibiotics
2.GFPmut3b
=4 Combinations

7. Insertion Sequence Terminators Terminators Registry Insertion Sequence
1.Double Terminator
Insertion Sequence
1.PyrD
2.AmyE

8. Protocols
Cloning,
Culturing,
Transformation,
Testing,

9. Cloning

10. Culturing and Transformation
This week we are planning to test culturing and transformation using integration vector.
If successful will linearise vector and test for linear integration

11. Have defined testing Constructs.
This week need to work out testing protocols,
Microscope training on Thursday!