1. Dnyanasadhana College, Thane.
Satish Pradhan
Dnyanasadhana College, Thane.
Department of Chemistry T.Y.B.Sc. Analytical Chemistry Paper-IV Sem-VI Size Exclusion Chromatography
By Dr.Bhagure G.R.
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2. Contents 3.3 Size Exclusion Chromatography (SEC) (02L)
3.3.1Introduction, Basic Principle, Applications of SEC
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3. Size Exclusion Chromatography
This separation method originated in 1959 at the Biochemical Institute in Uppsala, Sweden.
Size Exclusion Chromatography
Gel filtration
Gel permeation
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4. Separation of molecules according to Molecular size
Used for separation of proteins, polymeres, sugars in different type of fruit
Separation of molecules according to Molecular size
Large size molecule
Small size molecule
Introduction
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5. ex; agar, agarose,polyacryamide cross linked dextrin's
Basic Principles of Size Exclusion Chromatography
Stationary Phase
Gel molecules
ex; agar, agarose,polyacryamide cross linked dextrin's
Sample molecules
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6. Column Packing 2.
Gel
Size 5 to 10 um 1.
Polymer beads
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7. Applications of Size exclusion chromatography :1
Separation of High molecular weight compounds
ex. Proteins can be separated from amino acids 2
Homologue and oligomers can be separated from each other 3
Determination of molecular weight or distribution of the molecular weight 4
Desalting is a another applications which involves removal of salts and low molecular weight compound from macromolecules
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8. Advantages of Size exclusion chromatography :1
Method is very simple to perform 2
It provides conditions for maximum stability to very labile materials such as enzymes. 3
Gels can be reused 4
Useful in various industries like petroleum ,coating and varnishes and in the field of medicine
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9. Thank You For Kind Attention
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