1. Zerene Stacker
Bio 498/698

2. Outline: Review: Opening the Zerene Stacker Adding Files
Stacking Files
Properly Saving/Naming Compiled Images
Closing the Project

3. Zerene Stacker: Opening
*To open: Press the M3 button on the keyboard

4. Zerene Stacker

5. Zerene Stacker: Adding Files
To add a set of slices, go to File>Add File(s)
Find the desired file
‘Ctrl+a’ To select all files
Click ‘Add’
You can also select the files in Explorer and drag and drop into Zerene Stacker.

6. Zerene Stacker
All of the files have now been added

7. Zerene Stacker: Stacking Files
To stack the files into one compiled image:
On the toolbar, select ‘Stack’
Align & Stack All (PMax)

8. Zerene Stacker: Saving Compiled Images
After the images have finished stacking, save the compiled image
Go to File
Save Output Image(s)

9. Zerene Stacker: Naming Compiled Images
For the File name, the Stacker will choose one for you.
To name the compiled image correctly, click on one of the sliced images.

10. Zerene Stacker: Naming Compiled Images
This will change the file name.

11. Zerene Stacker: Naming Compiled Images
Change the last number to be ‘C’ or ‘comp’. ex: “GEWA1234_100mm_1to1_013” changes to “GEWA1234_100mm_1to1_comp”
File type should be ‘TIFF image’
The File Saving Parameters should be 8 bits RGB
Click ‘Save’

12. Zerene Stacker: End
Once the compiled image has successfully saved, click File->Close Project>”Yes, discard any unsaved work”