1. UV laser fluorophore options Fortessa HCI 5 Laser FACSAria Cell Sorter

2. BUV dyes
BUV 395
Trying to figure out a complicated color phenotyping panel?
You should really consider one of the BUV dyes.
BUV 395 CD4 stained PBMC
These dyes also have very little spillover into any other detectors
BUV 496
BUV 496 CD4 stained PBMC

3. Fixable Blue viability dye ThermoFisher
Blue Viability dye intensity in two UV detectors
-Works in both UV detectors
-I would not recommend also trying another
BUV dye at the same time
Compensation from blue viability dye into violet laser detectors
-”spills” into BV421 more than BV510
-Overall very manageable
Conclusion- Blue Viability Dye is a nice alternative to DAPI or
other viability stains in crowded multicolor panels.

4. DAPI and BV421
DAPI
alone
Background: DAPI has a very broad excitation range. Before the addition of the UV laser,
DAPI was collected in the BV421 channel off the violet laser.
Summary: Now you can detect DAPI off the UV laser and also simultaneously detect
a BV421 antibody off the violet laser.
DAPI plus CD4 BV421
”live” gate including BV421 CD4+ cells