1. Volume 44, Issue 5, Pages 603-609 (November 2003)
Comparison of Partial Urethral Replacement with Acellular Matrix versus Spontaneous Urethral Regeneration in a Canine Model 
Ahmed Shokeir, Yasser Osman, Mohamed El-Sherbiny, Mahmoud Gabr, Tarek Mohsen, Mahmoud El-Baz 
European Urology 
Volume 44, Issue 5, Pages (November 2003)
DOI: /j.eururo

2. Fig. 1
Examples of ascending urethrogram of the study group: (a) normal urethra before surgery; (b) normal appearance at 1 month.;(c) relative narrowing at 1 month; and (d) urethral diverticulum at 1 month.
European Urology  , DOI: ( /j.eururo )

3. Fig. 2
Acellular matrix before implantation showing intact fiber framework consisting of elastin and collagen. (H&E ×100).
European Urology  , DOI: ( /j.eururo )

4. Fig. 3
Examples of histopathological examination at different time points after implantation of acellular matrix: (a) at 1 week, there is infiltration of the graft by red blood cells and mononuclear cells (H&E ×100), (b) at 2 weeks, the epithelium developed in uniform lining covering the matrix border (H&E ×100), (c) at 2 weeks, some myofibroblasts stained with trichrome (Trichrome ×200), (d) at 3 weeks, the urethral lumen is completely covered by irregular epithelium; vessels of larger diameters in the matrix are apparent (H&E ×200), (e) at 3 weeks, the submucosa is filled with myofibroblasts infiltrating the matrix (α-actin ×200), (f) at 8 weeks, the epithelium is regular; good vessel supply in the submucosa is apparent (H&E ×200), (g) at 8 weeks, α-actin staining showing myofibroblasts in the vessel walls (α-actin ×200), and (h) at 20 weeks, the graft appears as normal tissue similar to surrounding host tissue.
European Urology  , DOI: ( /j.eururo )

5. Fig. 3
Examples of histopathological examination at different time points after implantation of acellular matrix: (a) at 1 week, there is infiltration of the graft by red blood cells and mononuclear cells (H&E ×100), (b) at 2 weeks, the epithelium developed in uniform lining covering the matrix border (H&E ×100), (c) at 2 weeks, some myofibroblasts stained with trichrome (Trichrome ×200), (d) at 3 weeks, the urethral lumen is completely covered by irregular epithelium; vessels of larger diameters in the matrix are apparent (H&E ×200), (e) at 3 weeks, the submucosa is filled with myofibroblasts infiltrating the matrix (α-actin ×200), (f) at 8 weeks, the epithelium is regular; good vessel supply in the submucosa is apparent (H&E ×200), (g) at 8 weeks, α-actin staining showing myofibroblasts in the vessel walls (α-actin ×200), and (h) at 20 weeks, the graft appears as normal tissue similar to surrounding host tissue.
European Urology  , DOI: ( /j.eururo )

6. Fig. 4
Example of histopathology of one of the control dogs at 8 weeks showing partial regeneration of the urethral mucosa with slight fibrosis in the subepithelial layer and normal smooth muscle fibres (H&E ×200).
European Urology  , DOI: ( /j.eururo )