1. Catalase Concentrations
Olivia Clements
Makenzie Nelson
Alexis Beyke
Lily

2. Experiment
An experiment was conducted to determine how the concentration of catalase affects a solution of H2O2.
One trial was run on each of the three different concentrations of catalase.
A logger pro was used to measure the pressure of the oxygen given off by the reaction.
Hypothesis: If the concentration of catalase affects the reaction rate, then the highest concentration of catalase (800 units/mL) will have a higher reaction rate than the lower concentrations (200 units/mL, 400 units/mL).

3. Materials Needed 200 units/mL catalase Lab Journal
Distilled Water
800 units/mL catalase
Sterilized Droppers
3 sterilized test tubes
3% Hydrogen Peroxide (H2O2)
5 sterilized beakers
Safety Equipment (Goggles, Gloves, Aprons, etc.)
Logger Pro™
Computer

4. Steps of the Experiment
Add 3mL of H2O2 to each of the three test tubes, then add 5mL of distilled water to each of those three test tubes.
Add 2 drops of 200 units/mL catalase into test tube 1.
Quickly cap the test tube with the measuring stopper and lightly agitate the solution while measuring the reaction rate using Logger Pro™ on the computer for 100 seconds.
Record Results in lab journal.
Add 2 drops of 400 units/mL catalase into test tube 2.
Repeat step 3 and record results in lab journal.
Add 2 drops of 800 units/mL catalase into test tube 3.

5. Results
Our results showed that the 800 units/mL concentration of catalase had a steeper slope, and therefore, a greater reaction rate, than the 200 and 400 units/mL solutions.

7. Citations
Chemistry for Biologists. (2009). Enzymes. [Accessed January 13, 2016]. Retrieved from
Project Lead The Way. (2014). Project Investigating Enzyme Action. [Accessed January 12, 2016]. Retrieved from