1. Good Lab Etiquettes & Procedures*
07/16/96
Good Lab Etiquettes & Procedures
Institute Members
With additional contributions from:
Mr. Wade Baggett, Ms. Janice Dodge (EH&S), Dr. Joan Hare, Mr. Ed Kirkland, Dr. Tim Logan, Dr. Claudius Mundoma, Mr. Rick Wang, and Dr. Michael Zawrotny
 2006 Thayumanasamy Somasundaram *

2. © 2006 Thayumanasamy Somasundaram*
07/16/96
Introduction
Scope
Reason
Instances of Improper Practices
No Mechanisms to Inform
Aim
Improve Safety
Update Current Procedures
11/16/2018
© 2006 Thayumanasamy Somasundaram *

3. © 2006 Thayumanasamy Somasundaram*
07/16/96
Overview
Supplement to FSU Safety Trainings
Review Lab Practices & Etiquettes
General & Wet Lab
Biological & Radioactive Samples
Help You Find & Get Things Done
Common Labs & Core Facilities
Computer Help & Contact Info
11/16/2018
© 2006 Thayumanasamy Somasundaram *

4. Emergency Information*
07/16/96
Emergency Information
Fire Extinguisher
Contact Info
First Aid
EXIT
11/16/2018
© 2006 Thayumanasamy Somasundaram *

5. © 2006 Thayumanasamy Somasundaram*
07/16/96
Contact Info
Env. Health & Safety
General:
Lab Safety:
Bio Safety:
Rad Safety:
Chem Safety:
General
Emergency: 911
Urgency: 311
Police:
Maintenance:
Information:
11/16/2018
© 2006 Thayumanasamy Somasundaram *

6. © 2006 Thayumanasamy Somasundaram*
07/16/96
Emergency Help
In Every Lab
In Hallway
Drench Hose
Eye Wash & Shower
11/16/2018
© 2006 Thayumanasamy Somasundaram *

7. © 2006 Thayumanasamy Somasundaram*
07/16/96
Emergency Help
In Hallway
In Hallway
Fire Pull Station
Fire Extinguisher
11/16/2018
© 2006 Thayumanasamy Somasundaram *

8. © 2006 Thayumanasamy Somasundaram*
07/16/96
General Lab Etiquette
No Food or Drink in Labs
Proper Attire & Footwear
Consider An Eye Wear
Work Under A Fume Hood
Cylinders Should be Strapped
Ice Machine for Samples
Working Late and Weekends
11/16/2018
© 2006 Thayumanasamy Somasundaram *

9. © 2006 Thayumanasamy Somasundaram*
07/16/96
Helpful People
Building & Common Lab Issues:
Wade Baggett or
Ed Kirkland (KLB 121 & 123)
Electronic, Electrical & Projector Issues:
Rick Wang (KLB 119)
11/16/2018
© 2006 Thayumanasamy Somasundaram *

10. © 2006 Thayumanasamy Somasundaram*
07/16/96
Wet Lab Etiquettes
Gloves Usage
Select Correct One
Nitrile, Latex, & Vinyl
Disposable Gloves Are Resistant But Not Impervious to All Chemicals
Use Only Inside the Lab
Discard Before Using Common Equipment
Change Often
11/16/2018
© 2006 Thayumanasamy Somasundaram *

11. © 2006 Thayumanasamy Somasundaram*
07/16/96
Proper Gloves Usage NO
YES NO NO
11/16/2018
© 2006 Thayumanasamy Somasundaram *

12. Sharps & Broken Glass Disposal*
07/16/96
Sharps & Broken Glass Disposal
Proper Sharps Disposal
Use Designated Units
Use Strong Lined Marked Box
Replace When 75% Full
Proper Broken Glass Disposal
Use Designated Unit
Good Lined Cardboard Marked Unit
11/16/2018
© 2006 Thayumanasamy Somasundaram *

13. Disposal Units (Ideal)*
07/16/96
Disposal Units (Ideal)
EH & S Can Provide Disposal Units
Sharps Disposal Unit
Glass Disposal Unit
Needles, Razors, Syringes, etc.,
Bottles, Beakers, Test Tubes, etc.,
11/16/2018
© 2006 Thayumanasamy Somasundaram *

14. © 2006 Thayumanasamy Somasundaram*
07/16/96
Wet Lab Etiquette
No Haz Chemicals in Drain or Sink
Chem Waste Containers from EH & S
Call EH & S For Pick-Up
No Haz Waste in Trash
Label Samples w/ Name, Date, & Chemical
11/16/2018
© 2006 Thayumanasamy Somasundaram *

15. Chemical Waste Containers*
07/16/96
Chemical Waste Containers
Bulk Waste Unit
Waste Disposal Unit
Name | Date
Chemical
Name | Date
Chemical
11/16/2018
© 2006 Thayumanasamy Somasundaram *

16. © 2006 Thayumanasamy Somasundaram*
07/16/96
Biological Samples
Bacterial Waste > Autoclavable Waste
Bacterial Tips > Autoclavable Waste
Autoclave When 75% Full
Autoclaved Waste > Regular Trash
Other Waste > Regular Trash
DO NOT Mix In Autoclave Bag
Consult with Dr. Joan Hare
11/16/2018
© 2006 Thayumanasamy Somasundaram *

17. © 2006 Thayumanasamy Somasundaram*
07/16/96
Radioactive Samples
Permission & Radiation Safety Training
PEF, Li, & Greenbaum Labs
Separate & Dedicated Areas
Aware of Contamination
Proper
Storage, Disposal of Waste, and Log
11/16/2018
© 2006 Thayumanasamy Somasundaram *

18. © 2006 Thayumanasamy Somasundaram*
07/16/96
Radiation Safety
Radiation Safety Training
Request Personal Radiation Monitor
Radioactivity & Radiation Safety
X-Ray Facility in KLB 410
11/16/2018
© 2006 Thayumanasamy Somasundaram *

19. Common Labs KLB 215 & KLB 515 Special Training Required*
07/16/96
Common Labs
KLB 215 & KLB 515
Special Training Required
Centrifuges: Rick Wang
Autoclaves: Wade Baggett
Micro fluidizer: Wade Baggett
French Press: Wade Baggett
11/16/2018
© 2006 Thayumanasamy Somasundaram *

20. © 2006 Thayumanasamy Somasundaram*
07/16/96
Common Labs
KLB 211, 212, 511, & 512
Ice Machines: Wade Baggett
Ultra colds: Rick Wang
Nano-pure: Rick Wang
Sorvall Speed Vac: Rick Wang
11/16/2018
© 2006 Thayumanasamy Somasundaram *

21. © 2006 Thayumanasamy Somasundaram*
07/16/96
Machine & Wood Shops
KLB 121
Wade Baggett
(4-1419)
Machining of Small Items
Common Repairs
User Training
KLB 123
Ed Kirkland
(5-1338)
Wood Cabinets
Book Shelves
Tables & Desks
11/16/2018
© 2006 Thayumanasamy Somasundaram *

22. © 2006 Thayumanasamy Somasundaram*
07/16/96
Electronics Shop
KLB 119
Rick Wang (5-3309)
Minor Electrical Problems
Electronics Troubleshooting
Small Instrument Repairs
11/16/2018
© 2006 Thayumanasamy Somasundaram *

23. Core: Computer Resources (CR)*
07/16/96
Core: Computer Resources (CR)
Director: Dr. Michael Zawrotny
Hardware & Core Software Installation
Scientific Software Help
Computer Connectivity & Virus Issues
, Web Page, & Printer Issues
Data Archiving Consultation
11/16/2018
© 2006 Thayumanasamy Somasundaram *

24. Computer Resources KLB Basement & Common Areas Webpage Maillistings*
07/16/96
Computer Resources
KLB Basement & Common Areas
Webpage
Maillistings
Send to a Specific List to “Subscribe” or “Unsubscribe”
11/16/2018
© 2006 Thayumanasamy Somasundaram *

25. Core: Physical Biochemistry Facility (PBF)*
07/16/96
Core: Physical Biochemistry Facility (PBF)
Equipment, Expertise, and Training
Circular Dichroism (CD)
Differential Scanning Calorimetry (DSC)
Isothermal Titration Calorimetry (ITC)
Stopped & Quenched Flow Systems
Dynamic Light Scattering (DLS)
UV-Vis & Fluorescence Spectrometers
11/16/2018
© 2006 Thayumanasamy Somasundaram *

26. Physical Biochemistry Facility*
07/16/96
Physical Biochemistry Facility
KLB except 312
Director: Dr. Claudius Mundoma
Facility Training Required
FSU Chem Safety Training
Multi-user Facility
PBF Info via Blackboard (Soon!)
Subscribe to “pbfusers” Mail Listing
11/16/2018
© 2006 Thayumanasamy Somasundaram *

27. Core: Protein Expression Facility (PEF)*
07/16/96
Core: Protein Expression Facility (PEF)
Large Scale Production of Proteins from Mammalian & Insect Cells
Equipment, Space, & Expertise Provided for Protein Expression
Graduate Student Training
Assistance for Protein Expression in E. Coli - Proper Bacterial Handling
Bio Safety Related Discussions
11/16/2018
© 2006 Thayumanasamy Somasundaram *

28. Protein Expression Facility*
07/16/96
Protein Expression Facility
KLB 312 A-G
Director: Dr. Joan Hare
Facility Training Required
FSU Bio Safety Training
Multi-user Self-contained Facility
Several Physically Isolated Rooms
Portions BSL2 Capable
11/16/2018
© 2006 Thayumanasamy Somasundaram *

29. Core: X-Ray Facility (XRF)*
07/16/96
Core: X-Ray Facility (XRF)
Macromolecular X-Ray Diffraction
Crystal Set-up & Documentation
Data Collection & Interpretation
3-D Stereo Viewing & Interpretation
Crystal Storage & Transportation
Synchrotron Scheduling & Training
11/16/2018
© 2006 Thayumanasamy Somasundaram *

30. © 2006 Thayumanasamy Somasundaram*
07/16/96
X-Ray Facility
KLB
Director: Dr. Thay “Soma” sundaram
Facility Training Required
FSU Radiation Safety Training
Multi-user Facility
Subscribe to “xrfusers” Mail Listing
11/16/2018
© 2006 Thayumanasamy Somasundaram *

31. IMB Computer Architecture*
07/16/96
IMB Computer Architecture X
11/16/2018
© 2006 Thayumanasamy Somasundaram *

32. Computer: Profile Size Errors*
07/16/96
Computer: Profile Size Errors
Good Profile Size
Set Small Cache in Internet Explorer & Firefox (~50 MB)
Don’t Store Files in Desktop
Store Files in D:\ or E:\ Drives
Remove or Archive Data Often (3 months)
Almost filled Profile
Filled-up Profile Area
11/16/2018
© 2006 Thayumanasamy Somasundaram *

33. © 2006 Thayumanasamy Somasundaram*
07/16/96
Printer Related
Default as B&W Printer (KLB 418)
Discreet in Printing Color (21¢ vs. 6¢)
PosterPS for Posters
(KLB 005)
Remove or Discard Your Print Job!!
11/16/2018
© 2006 Thayumanasamy Somasundaram *

34. © 2006 Thayumanasamy Somasundaram*
07/16/96
Contact Info
Building Problems:
Wade Baggett: (KLB 121) or
Ed Kirkland: (KLB 123) or
Rick Wang: (KLB 119)
Bio/Chem Safety Issues:
Thay Soma: (KLB 414)
Joan Hare: (KLB 304)
Computer Problems:
Michael Zawrotny: (KLB 415)
11/16/2018
© 2006 Thayumanasamy Somasundaram *

35. © 2006 Thayumanasamy Somasundaram*
07/16/96
Thanks
Feedback Welcome
Thanks for Attending
11/16/2018
© 2006 Thayumanasamy Somasundaram *