1. DNA Clean-Up Using MagNA Beads

3. Library Prep Using the “With-Bead” Method

4. Blunt-end repair Shearing the genomic DNA Adapter ligation Fill-in
Pre-hybridization PCR “with-beads”

5. Shearing the genomic DNA

6. Blunt-end repair

7. Blunt-end repair

8. Adapter ligation

9. Adapter ligation
IS7
INLINE INDEX
DNA
INLINE INDEX
IS8

10. Adapter ligation

11. Fill-in

12. Fill-in

13. Pre-hybridization PCR “with-beads”

14. Gene capture for target sequencing

15. Hybridization
Bind to beads and wash
Post-hybridization indexing PCR

16. Gene capture

17. Add 3 µL of the mixture to each of the empty tubes labeled Target enrichment – capture with sample name, and then add 7 µL of “PreH” sample (adjust according to conc., usually 100ng ~ 500ng in total) to each tube

18. IS7
INLINE INDEX
DNA
INLINE INDEX
IS8

20. HYB Baits Mix（19µL）
Lib Mix（10µL）
HYB Baits Mix
Lib Mix（10µL）
95 °C
65 °C
65 °C
60 °C
55 °C
50 °C
50 °C
5 min
5 min
300 min
300 min
300 min
300 min
forever

21. Bind to beads and wash
Please don’t stop the pcr program, we will still use it.
Open the hybridization machine before you do the experiment.

22. Bind to beads and wash

23. Bind to beads and wash

24. Post-hybridization indexing PCR

25. Adapter ligation P5
IS7
INLINE INDEX
DNA
INLINE INDEX
IS8 P7