1. Topic 3.6
Enzymes

2. 3.6.1 Define enzyme and active site
Enzymes are globular proteins that works as catalyst of chemical reactions.
They speed up chemical reactions without being altered themselves.
Conventionally we say the enzyme acts on the substrate (S) to yield a product (P)
ACTIVE SITE
Active site is the region on the surface of the enzyme to which substrate(s) bind and which catalyses the reaction (the conversion of the substrate to the product).
Sucrase
Substrate
Product
Without enzymes, many chemical processes would happen at a very slow rate in living organisms. By making some enzymes and not others, cells can control what chemical reactions happen in their cytoplasm.
Sucrase is an enzyme that binds sucrose and breaks the disaccharide into fructose and glucose.

3. 3.6.1 Define enzyme and active site

4. 3.6.2 Explain enzyme-substrate specificity

5. 3.6.3 Explain the effects of Temperature, pH and Substrate Concentration on enzyme activity
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9. 3.6.3 Define denaturation
Denaturation is a structural change in a protein that results in the loss (usually permanent) of its biological properties. Enzymes can be denatured by pH and Temperature.
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10. 3.6.5 Explain the use of lactase
in the production of lactose free milk