1. Multi-Disciplinary Senior Design
Flow Culture System
Multi-Disciplinary Senior Design
System Design Review
October 2, 2014
Collin Burkhart
Katelyn Busse
Michelle Garofalo
Robert Repetti
Morgan Stoessel
Sarah Tran
Team

2. Agenda CRs and ERs Functional Decomposition Morphological Chart
Benchmarking
Concept Generation/Pugh Analysis
Turning Point/Concept Selection
System Architecture
Alternative Concepts
Feasibility Analysis
FMEA
Risk Assessment
Project Plan
Deliverables
Questions

3. Essential Customer Requirements
Rob

4. Essential Engineering Requirements

5. Functional Decomposition
Kate

6. View Cells
Kate

7. Culture Cells
Kate

8. Control Environment
Michelle

9. Morphological Chart-1
Michelle

10. Morphological Chart-2
Rob

11. Morphological Chart-3 Morgan
Change Flow Media from Syringe to Peristaltic Pump

12. Benchmarking - Whole System
Kate

13. Benchmarking - Whole System
Kate

14. Benchmarking - Pumps
Morgan

15. Benchmarking - Materials
Collin

16. Benchmarking Shear Measurement and Control Requirements
Shear Range: 0-20 dynes/cm2 = 0-2.9X10-4 psi
Flow Range: mL/second
System Temperature: 25-37℃
Autoclave Temperature: Max 121℃
Strain Gauge
Load Transducer
Conversion Table
Can it handle autoclave and system temperatures? No
Yes
N/A
Can it measure a low force value?
No (0.2 psi)
No (0.1 psi)
Yes (Flow Sensor)
Can a display be made?
Is it cost feasible? <500
Yes ($160)
No ($500)
Yes (Free)
Rob

17. Concept Generation-Pugh Analysis
Sarah
Fix Design Names - more clear “incubator and peristaltic pump” “sensors and positive displacement pump”
Totals - recheck math!

18. Turning Point
Kate and Morgan

19. Concept Selection Collin explain reasoning behind choosing this one
No Cost, Known Consistent Environment, No extra labor, No need for user calibration, Able to sterilize itself

20. System Architecture Incubator Collin CO2 Heat Water Pan Cells Filter
Cell Chamber
Microscopes
Alternate Reservoir
Media Reservoir
Pump
Media
Architecture
Color
Structure
Energy
Information
Materials
Electricity
Media Color
Collin
Flow Rate

21. Alternative Cell Viewing Concepts
Keep Attached for Viewing
Have to move microscope next to incubator
Extra length of hose to take out of incubator and set on microscope
Carry total system on a tray
Detach from System for Viewing
Increase risk for contamination
Close off chamber
clamps on tubing (2 on each side)
Valves
Caps (like flasks currently use)
Collin

22. Alternative Subsystem Concepts
Rob
Media Circulation
System

23. Feasibility Analysis-Pumps
Syringe Pump
Peristaltic Pump
Morgan
types of pumps used in experiments previously done, talk about flow rates, talk about features

24. Feasibility Analysis-Shear Stress/Flow Rates
Values used for calculation
μ=0.78x10^-3 Ns/m^2
b=0.06m
a=0.0125m
T=2 N/m^2
Flow Rate
.016 L/s
Dependent on Chamber Size
T=shear stress
μ=viscosity of the liquid
Q=flow rate
b=channel width
a=½ channel height
Morgan-source the article, check on equations
Kojima, Naoya, Mitsuru Shiota, Yoshito Sadahira, Kazuko Handa, and Sen-itiroh Hakomori. "Cell Adhesion in a Dynamic Flow System Compared to Static System."The Journal of Biological Chemistry (1992): Web. 1 Oct
Wang, Chong, Hao Lu, and Martin Alexander Schwartz. "A Novel in Vitro Flow System for Changing Flow Direction on Endothelial Cells." Journal of Biomechanics 45.7 (2012): ELSEVIER. Web. 1 Oct

25. Feasibility Analysis - Materials
Polycarbonate (PC)
Several buying options
Variety of readily available sizes
Low cost
Polymethylpentene (PMP)
Very limited buying options
Sparse size options
Far more expensive than PC
Collin - TAP Plastics, 1 in2 to several feet2, 1/32” thick to ½” thick, 12 1ft2 sheets and 6 6”2 sheets at ⅛” thick for only 17% of “other materials” budget ($87)
Thickest sheet only .02” thick (.5mm), a single 1ft2 sheet is nearly 8% of the budget

26. Feasibility Analysis-Budget
Sarah

27. FMEA
Sarah

28. FMEA Cont.
Sarah

29. Risk Assessment Lowered Risks Risk 8-Sensor Knowledge
Risk 17-Media contamination during microscope viewing
Increased Risks
Risk 18-Media contamination during exchange
“Still at Large” risks
Risk 15 and 16-Shear stress measurement
Morgan-talk about projected risk in script

30. Project Plan: MSD-I
Michelle

31. Deliverables Proof of Concept Consultation with Material Experts
Further Engineering Analysis
Simulations/3D Models
Prototyping of Subsystems
Consultation with Material Experts
Dr. Man
Dr. Gaborski
Mr. Hanzlik
Michelle

32. Questions, Comments, or Concerns? Thank you!
Everyone

33. Back-Up Slides House of Quality:
House of Quality - Michelle

34. Back Up Slides
Risk Table - Morgan

35. Back Up Slides
Sarah - Outline of Each Alternative Concepts with details