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The expected molecular weight for GST-Nup42 AA 1-382 is 67,144 Da
Purification of GST-Nups from E. coli via Ligand-Affinity Chromatography The expected molecular weight for GST-Nup42 AA is 67,144 Da WCE MSS BSA kDa MW +IPTG MSS 5 ul 50ul 1ug 2ug 4ug 200 116 97 68 BSA GST-Nup42 AA 1-382 44 31
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Affinity-Capture of Karyopherins from Yeast Lysates Using GST-Nups as Bait
The expected molecular weight of GFP-Mex67 is 96 kDa The concentration of protein in the GFP-only HSS cytosol was [ ] The concentration of protein in the GFP-Kap HSS cytosol was [ ] 200 116 97 68 44 31 kDa MW MgCl2 eluates GST-Nup116 FG domain GST-Nsp1 K>R GFP-Kap GFP SDS eluates yeast HSS Kaps
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Charting the path that karyopherins take across the nuclear pore complex
Cytoplasm Nuclear Pore Complex Nup159 Nup159 Nup42 Nup116h Nup116h Nup116s Nup116s Nup42 Nup57 Nup100s Nup100h Nup100h Nup100s Nup57 Nup49 Nsp1s Nsp1h Nsp1h Nsp1s Nup49 ER lumen Nup49 Nsp1h Nsp1h Nsp1s Nsp1s Nup49 Nup57 Nup145h Nup145h Nup145s Nup145s Nup57 Nup60 Nup1h Nup1h Nup1s Nup1s Nup60 Nup2 Nup2 Nucleoplasm Nup116h [Turn the box green as shown, if your GFP-Kap bound to the FG domain in the BEAD HALO assay]
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