1. Metabarcoding Presentation
By: Anna, Sharleen, Maureen

2. Type of Article Review article No methods or results sections
Broad overview of what was found when the sequencing analyzed
Acknowledgment section
Lots of sources being cited throughout the article

3. Purpose Realized the large diversity of soil parasitic protists
Many ways to help find this diversity
Used to just have people with taxonomic expertise now more advanced technology
HTS (high-throughput sequencing)
There continues to be improvements on the sequencing technology
- Finding this diversity helps ecological functions and the ecosystem

4. Metabarcoding Challenges
The majority of the few studies investigating the community structure of soil protists applied time-consuming microscopic determinations that require profound taxonomic expertise.
Another challenge is finding a diversity of soil protists similar to that of bacteria and having their sequence closely match it to that of parasites
There are little studies known about the community structure of soil protists so the studies made on soil protists require profound taxonomic expertise

5. Information that can be applied to the Research Question
(How to extract DNA and what primer we should use to amplify it?)
Problems:
- “Close attachment of many protists to soil particles eliminates straight forward extraction.”
-“Designing universal protist-specific primers is impossible”.
-The diversity of the protists is just too great and too different -

6. Eukaryotic Tree of Life

8. Information that can be applied to the Research Question
(How to extract DNA and what primer we should use to amplify it?)
Solutions:
-The trick is using “multi-methodological approaches” to link
-morphological
-molecular
-functional
information of distinct parasites,

9. Information that can be applied to the Research Question
So we can:
-decipher patterns
-determine identities
-evaluate the effects of the treatments on the different protists.
(How to extract DNA and what primer we should use to amplify it?)

10. Information that can be applied to the Research Question
(How to extract DNA and what primer we should use to amplify it?)
We can use:
- Classical cultivation based efforts
- Manually mined DNA (Amplicon) - based HTS studies
- Phylogenetic analyses
- PCR free Metatranscriptomic and Metagenomic approaches
*Most reliable for soil protist communities*
- pH Analyses

11. Our Questions Vocabulary: Concepts:
Phylogenetic-“relating to the evolutionary development and diversification of a species or group of organisms, or of a particular feature of an organism.”
Amplicon-“a piece of DNA or RNA that is a source and/or product of natural or artificial amplification or replication events.”
Monophyletic- “(of a group of organisms) descended from a common evolutionary ancestor or ancestral group, especially one not shared with any other group.”
Concepts:
HTS ( the detailed process and the meaning of the results found)

12. Bibliography
Ph Picture:
Stentor and Tetrahymena gifs:
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Ciliate Morphology and Classification Pictures:

13. Bibliography Title Gif:
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